Channel: Plant physiology, biochemistry and molecular biology http://www.yrdzwkx.com EN-US http://www.yrdzwkx.com/EN/1009-7791/current.shtml http://www.yrdzwkx.com 1009-7791 http://www.yrdzwkx.com/EN/10.3969/j.issn.1009-7791.2023.05.001 D27, D14, DAD2 and SMAX1-LIKE4 compared to CK and TIS-108 [a triazole-type strigolactone(SL)-biosynthesis inhibitor] treatment, whereas, D27, D14 and DAD2 were down-regulated by TIS-108 treatment, the expression of SMAX1 was inhibited by SL, while TIS-108 treatment induced the expression of SMAX1. The axillary buds of tobacco are developed from three hyphal regions of the axillary meristem, while different plant growth regulators have different effects on the axillary buds of tobacco. BR can promote the elongation of the axillary buds of tobacco, while SL can inhibit the elongation of the axillary buds, and the genes related to the metabolic pathway may play an important regulatory role in the elongation of the axillary buds of tobacco.]]> http://www.yrdzwkx.com/EN/10.3969/j.issn.1009-7791.2023.05.002 Cinnamomum burmanni leaves from Meizhou, Guangdong Province. At the same time, the antibacterial activities of the compounds were determined by microplate method, and their anti-inflammatory activities were determined by LPS-induced BV2 cells. The results showed that 25 compounds were isolated and identified from the 95% ethanol extracts of C. burmanni leaves, including flavonoids (1–3), lignans (4–6), simple phenylpropanoids (7–16), coumarins (18), benzoquinones (20–21), sesquiterpenes (22–24) and indole alkaloids (25). Except for compounds 1, 2, 5 and 18, all of them were isolated from C. burmanni for the first time. The results of antibacterial experiments showed that compounds 20 and 21 showed good antibacterial activity against Staphylococcus aureus, with MIC of 32 μmol·L^–1, while MBC of compound 20 was 64 μmol·L^–1, and MBC of compound 21 was 32 μmol·L^–1. The results of antibacterial kinetics also showed that compound 21 had better antibacterial activity than compound 20. The results of anti-inflammatory experiments showed that compounds 5, 6 and 7 could inhibit the production of NO in BV2 cells induced by LPS, and showed good anti-inflammatory activity, the IC₅₀ values were 7.85 μmol·L^–1, 24.97 μmol·L^–1 and 7.49 μmol·L^–1, respectively.]]> http://www.yrdzwkx.com/EN/10.3969/j.issn.1009-7791.2023.05.003 Pueraria starch were prepared by extracting and enzymatic modification or annealing, then blending and soaking the modified starch with the fresh extract produced during the extraction process. The physical and chemical properties of Pueraria starch treated with two modified soaking methods were compared. The results showed that the content of amylose in the starch was decreased, the solubility and water absorption index were increased, and the blending property was improved by the two modified soaking treatments. The two treatment methods also increased the initial temperature (T0), peak temperature (Tp), and final temperature (Tc) of Pueraria starch, and reduced the gelatinization temperature range (ΔT) and enthalpy value(ΔH), which indicated that the modified Pueraria starch was more stable to low temperature conditions and more prone to gelatinization under high temperature conditions. Both treatments significantly increased the content of Puerarin in Pueraria starch. And the digestive characteristics were characterized by a decrease in the content of fast digestible starch (RDS), while an increase in the content of slow digestible starch (SDS), which indicated that two modified soaking methods of Pueraria starch would have a positive effect on increasing satiety, reducing blood sugar, and protecting intestinal health.]]> http://www.yrdzwkx.com/EN/10.3969/j.issn.1009-7791.2023.05.004 Prunus pseudocerasus × incisa. The results showed that the chlorophyll content decreased first and then increased as the stress temperature increased, and the SOD activities, the contents of MDA and the soluble protein increased first and then decreased. CAT, POD activities showed an upward trend. The contents of chlorophyll, soluble protein, soluble sugar, MDA, and POD, SOD activities were presented significant differences along with the duration of stress prolongs under the same temperature stress. By means of principal component analysis showed that POD activity, SOD activity, and contents of chlorophyll, soluble protein and soluble sugar were the main physiological index to high temperature stress.]]> http://www.yrdzwkx.com/EN/10.3969/j.issn.1009-7791.2023.05.005 Camellia drupifera seedlings after ⁶⁰Co-γ irradiation, the authors did a ⁶⁰Co-γ by 0 Gy, 40 Gy, 50 Gy, 60 Gy and 70 Gy irradiation of seeds from two excellent materials C. drupifera ‘Fanlong 3’ and ‘Fanlong 5’ and measured chlorophyll content, soluble sugar content, soluble protein content, peroxidase (POD), superoxide dismutase (SOD) in leaves. The result showed that the influence on physiologic index of C. drupifera of ⁶⁰Co-γ irradiation by various kinds dose was different. The chlorophyll content of 50 Gy group of ‘Fanlong 3’ and ‘Fanlong 5’ were significantly lower than those of other groups (P<0.05). The change tends of soluble sugar content on ‘Fanlong 3’ and ‘Fanlong 5’ were different which showed that the 60 Gy of ‘Fanlong 3’ was relatively low and the 70 Gy of ‘Fanlong 5’ was relatively low. The change tends of soluble protein content on ‘Fanlong 3’ and ‘Fanlong 5’ were the same which showed that the soluble protein contents of 60 Gy treatment were relatively low. The change tends of antioxidant enzyme activity on ‘Fanlong 3’ and ‘Fanlong 5’ were different. The SOD activity of high dose group (60 Gy, 70 Gy) was relatively low and the POD activity of 40 Gy treatment was relatively high in ‘Fanlong 3’. The SOD activity of control group was relatively low and that of 70 Gy treatment was relatively high in ‘Fanlong 5’. The POD activity of 60 Gy treatment was relatively low and that of 50 Gy treatment was relatively high in ‘Fanlong 5’.]]> http://www.yrdzwkx.com/EN/10.3969/j.issn.1009-7791.2023.05.006 Begonia masoniana pollen, a liquid culture method was used to investigate the effects of sucrose, boric acid, PEG-4000, calcium chloride concentration, and different cultivation times on pollen tube growth through single factor experiments. The results showed that the optimal medium for pollen germination was sucrose 5%, boric acid 0.01%, PEG-4000 5%, calcium chloride 0.003%. The optimal observation time was 120 min. The pollen germination rate of B. masoniana was 90.86%. The length of the pollen tube was 511.51μm. Our results could provide reference for pollen research and breeding of other begonias.]]>