Subtropical Plant Science ›› 2009, Vol. 38 ›› Issue (03): 21-23.DOI: 10.3969/j.issn.1009-7791.2009.03.005

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Studies on Influencing Factors of Plantlet Regeneration of Stem Segments in Pedicel of Oncidium hybridum

LI Li, LUO Jun-qin, XU Jian-guo, NIE Zhen-peng, KE Fu-zhi, SUN Jian-hua   

  1. Citrus Research Institute of Zhejiang Province, Huangyan 318020, Zhejiang China
  • Received:2009-06-01 Online:2009-09-10 Published:2009-09-10

文心兰花梗茎段植株再生培养的影响因子研究

李丽,罗君琴,徐建国,聂振朋,柯甫志,孙建华   

  1. 浙江省柑橘研究所, 浙江 黄岩 318020
  • 作者简介:李丽(1976-),女,浙江黄岩人,助理研究员,本科,从事园艺植物组织培养及生物育种研究。
  • 基金资助:

    国家现代农业(柑橘)产业体系专项经费(MATS-NYCYTX-07)资助

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Abstract: The effects of minimal medium,categories and concentration of plant hormone on the induction,proliferation and rooting cultivation of multiple shoots of stem segments in pedicel of Oncidium hybridum were studied by orthogonal experiment.The results showed that 1/2 MS medium was the best minimal medium for the growth of multiple shoots,the best induction medium was 1/2 MS+6-BA 5.0 mg/L+NAA 0.5 mg/L+sugar 20 g/L,the best proliferation medium was 1/2 MS+6-BA 3.0 mg/L+NAA 0.5 mg/L+sugar 30 g/L,and the best rooting cultivation medium was 1/2 MS+IBA 0.05 mg/L+NAA 0.3 mg/L+banana slurry 100 g/L+sugar 20 g/L.

Key words: Oncidium hybridum, orthogonal experiment, stem segments in pedicel, plantlet regeneration

摘要： 应用正交试验设计法研究基本培养基、植物生长调节剂种类及浓度对文心兰花梗茎段芽再生丛生芽诱导、增殖和生根培养的影响。筛选出最佳基本培养基为1/2 MS;最佳诱导培养基为1/2 MS+6-BA 5.0 mg/L+NAA 0.5 mg/L+蔗糖20 g/L;最佳增殖培养基为1/2 MS+6-BA 3.0 mg/L+NAA 0.5 mg/L+蔗糖30 g/L;最佳生根培养基为1/2 MS+IBA 0.05 mg/L+NAA 0.3 mg/L+香蕉泥100 g/L+蔗糖20 g/L。

关键词: 文心兰, 正交试验, 花梗茎段, 植株再生

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