Abstract: Dioscorea bulbifera virus-free plantlets were treated with different concentrations of NaCl to study the effects of salt stress on their growth and related physiological and biochemical indices.The results showed that the growth of Dioscorea bulbifera virus-free plantlets was markedly retarded under salt-stress.With the increasing of concentration,the total chlorophyll content and the activity of SOD decreased,while the contents of malonaldehyde (MAD) and proline increased.Moreover,under the salt-stress conditions,the content of proline in leaves of Dioscorea bulbifera virus-free plantlets had a significantly positive correlation to the content of MDA,but a significantly negative correlation to the total chlorophyll content and the activity of SOD.It was concluded that the changes of proline content in leaves could be used as a physiological index to reflect the hurt degree of Dioscorea bulbifera virus-free plantlets under salt-stress.

Key words: Dioscorea bulbifera, virus-free plantlets, salt-stress, growth, physiological and biochemical indices

摘要： 通过在培养基中添加不同浓度NaCl,探讨盐胁迫对黄独脱毒苗生长及若干生理生化指标的影响。结果表明,在盐胁迫条件下,黄独脱毒苗的生长受到明显抑制,叶片中总叶绿素含量、SOD活性下降,丙二醛含量增加,脯氨酸大量积累;随盐胁迫强度的加大,对试管苗生长及生理生化指标的影响相应加剧;在盐胁迫下,黄独脱毒苗叶片脯氨酸含量与丙二醛含量呈极显著正相关,而与叶绿素含量和SOD活性呈极显著负相关。因此,盐胁迫下叶片中脯氨酸含量的变化可作为黄独脱毒苗受害程度的主要生理鉴定指标。

关键词: 黄独, 脱毒苗, 盐胁迫, 生长发育, 生理生化指标