Subtropical Plant Science ›› 2008, Vol. 37 ›› Issue (04): 18-21.

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Simultaneous Determination of Four Kinds of Cytokinin Components in Arabidopsis thaliana by High Performance Liquid Chromatography

TONG Jian-hua1,2, ZHAO Wen-kui3, ZHANG Xue-qin1,2, LIANG Yan-ping1   

  1. 1. Hunan Provincial Key Laboratory of Phytohormone and Growth Development, Hunan Agricultural University, Changsha 410128, Hunan China;
    2. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, Hunan China;
    3. Property Administrative of Hunan Agricultural University, Changsha 410128, Hunan China
  • Received:2008-04-25 Online:2008-11-10 Published:2008-11-10

拟南芥中4种细胞分裂素的高效液相色谱法测定

童建华1,2,赵文魁3,张雪芹1,2,梁艳萍1   

  1. 1. 湖南农业大学 湖南省植物激素与生长发育重点实验室, 湖南 长沙 410128;
    2. 湖南农业大学 生物科学技术学院, 湖南 长沙 410128;
    3. 湖南农业大学 资产处, 湖南 长沙 410128
  • 作者简介:童建华(1970-),女,湖南桃江人,实验师,硕士研究生,从事植物学研究。

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Abstract: The study was conducted to establish the methods which to detect the contents of four kinds of cytokinin components (Z, ZR, 2ip and 2ipr) in Arabidopsis thaliana simultaneously by high performance liquid chromatography (HPLC).Results showed that they could be determined precisely in Arabidopsis thaliana by using reversed phase column Waters C18(4.6×250 mm, 5 μm), at 35℃, with acetonitrile and triethylamine buffer as mobile phase, and at flow speed 1 ml/min by gradient elution and with 270 nm.The detectable limitation of this method was 0.001 μg/ml.

Key words: high performance liquid chromatography, Arabidopsis thaliana, cytokinin components

摘要： 应用高效液相色谱法同时测定拟南芥中4种细胞分裂素组分玉米素(Z)、玉米素核苷(ZR)、6-r,r-二甲基烯丙基氨基嘌呤(2ip)和6-r,r-二甲基烯丙基氨基嘌呤核苷(2ipr)含量。结果表明,采用反相色谱柱Waters C18柱(4.6×250 mm,5 μm),在35℃以乙腈和三乙胺缓冲液为流动相梯度洗脱,流速1 ml/min,在270 nm处能准确检测出拟南芥中4种细胞分裂素组分的含量,检测限达0.001 μg/ml。

关键词: 高效液相色谱法, 拟南芥, 细胞分裂素组分

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