Subtropical Plant Science ›› 2007, Vol. 36 ›› Issue (02): 13-14.

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Study on Genetic Stability of Cymbidium goeringii Seedlings in Vitro by ISSR Markers

GAO Li, YANG Bo, LI Hong-lin   

  1. Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, Hubei China
  • Received:2006-11-16 Online:2007-06-10 Published:2007-06-10

叶艺春兰组培苗遗传稳定性的ISSR研究

高丽,杨波,李洪林   

  1. 中国科学院 武汉植物园, 湖北 武汉 430074
  • 作者简介:高丽(1979-),女,河南南阳人,硕士,从事植物生物技术和分子生物学研究。
  • 基金资助:

    湖北省自然科学基金(2004ABA119)

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Abstract: Inter-simple sequence repeats (ISSR) markers were used to investigate genetic stability for clonal plants of Cymbidium goeringii with verge line pattern leaves.The results showed that the culture clonal plants appeared without variations as compared with their original plants in substance of heredity and had no difference on the level of DNA.

Key words: Cymbidium goeringii, seedling in vitro, ISSR, genetic stability

摘要： 采用简单重复序列间扩增(ISSR)分子标记技术,对来自叶艺春兰同一母株并经多次无性繁殖获得的组培苗进行基因组DNA分析。研究结果表明,在多次继代培养过程中,组培苗遗传物质稳定,在DNA水平上未见明显差异,保持了母株的特性。

关键词: 春兰, 组培苗, ISSR, 遗传稳定性

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