Abstract: The terminal bud of Rhododendron simsii was used as explants to study the influence on establishing an aseptic strain by the different color of flowers, the different disinfect time and the different hormone combinations in medium.The results showed that the surface disinfection of 0.1% HgCl₂ for 10 minutes was best for the explants.Under the same disinfecting condition, the ratio of pollution of white flower type was lower than that of red-flower type, the ratio of browning of red-flower type was lower than that of white-flower type.The initial culture medium with high concentration of ZT resulted in low browning ratio of the explants.

Key words: Rhododendron simsii, aseptic strain

摘要： 以二乔杜鹃顶芽为外植体,探讨不同花色类型、不同消毒时间、培养基中不同生长调节剂组合对其无菌株系建立的影响。结果表明,以0.1%升汞作表面消毒剂,顶芽外植体的最适消毒时间为10 min;在相同消毒条件下,白花类型的污染率低于红色类型,红花类型外植体的褐变率低于白花类型;含较高浓度ZT的初代培养基中外植体的褐变率较低。

关键词: 二乔杜鹃, 无菌株系