Abstract: Effects of plant growth regulators on multiplication of cespitose buds from Asparagus officinalis new species ‘Jinling’ were studied.The result indicated that the admixture of NAA, 6-BA and KT gave good result for multiplication of cespitose buds.Taking the stem apex as explant, after 30 d, there were 26.3 cespitose buds per bottle when cultured in MS+NAA 0.1 mg/L+6-BA 0.2~0.3 mg/L+KT 0.1 mg/L;taking the mid stem as explant, after 30 d, there were 33.5 buds per bottle when cultured in MS+NAA 0.1 mg/L+6-BA 0.3~0.4 mg/L+KT 0.1 mg/L;taking the stem base as explant, there were 43.1 buds per bottle when cultured in MS+NAA 0.1 mg/L+6-BA 0.3~0.5 mg/L+KT 0.1 mg/L.The stem base was the optimum explant for rapid propagated.

Key words: Asparagus officinalis, tissue culture, cespitose buds, multiplication

摘要： 本试验以芦笋新品种"津宁"为材料,探讨植物生长调节剂对不同外植体产生丛生芽的效应。试验结果表明,NAA、6-BA和KT三者配合使用有利于丛生芽的增殖:以茎尖为增殖外植体时,培养基用MS+NAA0.1 mg/L+6-BA0.2~0.3 mg/L+KT 0.1 mg/L,30 d时平均每瓶可以产生20.5个丛生芽;以茎中段为外植体时,培养基用MS+NAA 0.1 mg/L+6-BA 0.3~0.4 mg/L+KT 0.1 mg/L,30 d时平均每瓶可产生33.5个丛生芽;以茎基为外植体时,培养基用MS+NAA 0.1 mg/L+6-BA 0.3~0.5 mg/L+KT 0.1 mg/L,30 d时平均每瓶可以产生43.1个丛生芽。进行试管苗工厂化生产时,增殖外植体以茎基为最佳。

关键词: 芦笋, 离体培养, 丛生芽, 增殖