Subtropical Plant Science ›› 2001, Vol. 30 ›› Issue (02): 1-5.

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Effects of NAA on the activity of IAAO and rooting of Eucalyptus cuttings

HUANG Zhuo-lie1, Li Ming1, TAN Shao-man2, LIN Shao-xiang1, LONG Teng3, YANG Guo-qing3   

  1. 1. College of Biotechnology, South China Agricultural University, Guangzhou 510642, Guangdong China;
    2. College of Forestry, South China Agricultural University, Guangzhou 510642, Guangdong China;
    3. Leizhou Forestry Institute of National Forestry Bureau, Zhanjiang 524348, Guangdong China
  • Received:2001-02-08 Online:2001-06-10 Published:2001-06-10

NAA处理桉树插条后IAAO活性与生根的关系

黄卓烈1,李明1,谭绍满2,林绍湘1,龙腾3,杨国清3   

  1. 1. 华南农业大学 生物技术学院, 广东 广州 510642;
    2. 华南农业大学 林学院, 广东 广州 510642;
    3. 国家林业局 雷州林业科学研究所, 广东 湛江 524348
  • 作者简介:黄卓烈(1950-),男,广东廉江人,教授,硕士,留学回国人员,从事生物化学的教学与科研工作。
  • 基金资助:

    广东省重点攻关项目(99M04201G)

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Abstract: Eucalyptus urophylla Blade MLA clone (MLA) is a species that is difficult to root and E. urophylla Blade U6 clone (U6) and E.ABL 12 W5 clone (W5) are two species that are easy to root. The activity of indoleacetic acid oxidase (IAAO) in MLA was higher than that in U6 and W5. After treated with naphthylacetic acid (NAA), the activities of IAAO varied and the contents of soluble protein increased regularly at different stages of rooting of Eucalyptus cuttings. The relationship between IAAO and rooting of Eucalyptus cuttings was also discussed.

Key words: indoleacetic acid oxidase, Eucalyptus, rooting of cuttings

摘要： 尾叶桉MLA无性系(简称MLA)为难生根植物,尾叶桉U6无性系(简称U6)和刚果12号桉W5无性系(简称W5)为相对易生根植物。MLA插条内的吲哚乙酸氧化酶(IAAO)活性较U6、W5的高。用萘乙酸(NAA)处理桉树插条后,在扦插生根的不同阶段,插条内IAAO活性呈现规律性变化;可溶性蛋白质含量呈上升趋势。本文讨论了IAAO与桉树插条生根的关系。

关键词: 吲哚乙酸氧化酶, 桉树, 扦插生根

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