Abstract: The compartmentation distribution of polyphenol oxidase (PPO), peroxidase (POD) and catalase (CAT) activities in Flammulina velutipes appeared. The activity levels were the lowest at the cap, high at the top of stipe, higher at the middle of stipe, and the highest at the bottom of stipe. Flammulina velutipes were treated with thiourea, sodium sulfite, Ca²⁺, and at high CO₂, low O₂ and low temperature, the activities of PPO,POD and CAT were inhibited. High O₂, H₂O₂ and high temperature enhanced the activities of PPO, POD and CAT. High N₂ could not inhibit the activities of them.

Key words: Flammulina velutipes, enzymic browning, regulation, compartmentation distribution of enzyme activity

摘要： 金针菇的多酚氧化酶(PPO)、过氧化物酶(POD)和过氧化氢酶(CAT)活性在全株中呈现区域化分布。菌盖的酶活性最低,菌柄上部酶活性稍高,菌柄中部较高,菌柄下部活性最高。用硫脲、亚硫酸盐、CaCl₂、低CO₂、低温等方法处理金针菇,对以上三种酶的活性均有抑制作用。高0₂、H₂O₂和温度的提高均促进三种酶活性。高N₂(减压后充N₂)不能抑制三种酶活性。

关键词: 金针菇, 酶促褐变, 调控, 酶活性区域分布