Abstract: The regeneration of bud clumps was adopted to establish a rapid technique system of propagation of Dendrobium nobile in this experiment using the Orthogonal Design. The main contents focused on the following aspects, such as the kinds of the basic culture medium, 6-BA, NAA and KT. Through above research, we tried to find the main influence factors and to build up the superior regeneration system of Dendrobium nobile. The result showed that the main factors of multiplication of bud clumps were the 1/2 MS basic culture medium, 6-BA and KT. The optimum medium was 1/2 MS + 6-BA 1.0 mg/L + NAA 1.0 mg/L + KT 0.5 mg/L + sugar 30.0 g/L + agar 7.0 g/L + coconut juice 150.0 ml/L, pH 5.4. In addition, the much fitter multiplication explants of Dendrobium nobile was 1.5 cm stem-segment with node, with the density of 3 seedlings every bottle.

Key words: Dendrobium nobile, bud clumps, tissue culture and rapid propagation, orthogonal design

摘要： 本试验采用正交设计，探讨春石斛组培以丛生芽途径进行快速繁殖的方法。研究主要集中在不同基本培养基类型、不同生长调节剂配比及不同添加物等因素对丛生芽增殖的影响，从中筛选最优技术参数组合，提高丛生芽增殖系数，建立春石斛最优再生体系。结果表明：影响丛生芽增殖的显著因子分别是基本培养基、6-BA、KT；最适宜的培养基配方是1/2 MS + 6-BA 1.0 mg/L + NAA 1.0 mg/L + KT 0.5 mg/L +蔗糖30.0 g/L +琼脂7.0 g/L +椰汁150.0 ml/L，pH 5.4。此外，春石斛丛生芽增殖的外植体以1.5 cm带节茎段、接入密度每瓶3株较适宜。

关键词: 春石斛, 丛生芽, 组培快繁, 正交设计