Abstract: ³H-leucine label method and fluorescein-fluoreceinase method were used to determine the rate of protein synthesis, the content of ATP in the soybean plumular axes pretreated with PVA (polyvinyl alcohol) and PEG (polythylene glycol-6000) and in the control during low-temperature imbibition and germination process. The results showed that the vigour and cold resistance of soybean seeds were closely related to the protein synthesis and ATP content.The ATP content and the synthesis of protein in the control seeds during low temperature imbibition stage was restrained, the ATP content in the plumular axes during germination stage markedly decreased,the protein syn thesis increased slowly. On the contrary, the ATP level in soybean plumular axes pretreated with PVA and PEG during low temperature imbibi tion was higher than that of the control; the protein synthesis at germination stage significantly increased, the ATP was more rapidly consumed by the plumular axes. The results suggest that the high rate of protein synthesis, the stronger ATP synthesis and consumption are the main causes for the stronger vigour and cold resistance of soybean seeds.

Key words: Protein synthesis, ATP content, Low temperature imbibi-tion, Soybean seed

摘要： 在低温吸胀阶段,经PVA(聚乙烯醇)和PEG(聚乙二醇6000)预处理的大豆胚轴蛋白质合成和ATP含量均比对照高。在萌发阶段,胚轴生长增快,蛋白质合成明显加快,ATP迅速被消耗,而对照胚轴则相反。试验结果表明,预处理大豆种子萌发和生长与其蛋白质合成、ATP水平和消耗能力有密切关系。

关键词: 蛋白质合成, ATP含量, 低温吸胀, 大豆种子