The spore sterile culture and routine propagation of Asplenium nidus were studied respectively. The results showed that the maximum rate of spore germination was 66.7% in the spore sterile culture; the sporophyte formation was almost impossible in solid culture mediums until the prothallus were cultured for 2 weeks in 1/2MS solution. In contrast, the sporophyte formation was easy in the spore routine propagation. When the spores were applied to soil by 0.02 g per pots, one gram of spores produced over 4 000 sporophytes. The spore routine propagation was simpler and less cost than the spore sterile culture. So the spore propagation is more suitable for the scale production with spores of A. nidus.