Subtropical Plant Science >

2009 , Vol. 38 >Issue 03: 21 - 23

DOI: https://doi.org/10.3969/j.issn.1009-7791.2009.03.005

Studies on Influencing Factors of Plantlet Regeneration of Stem Segments in Pedicel of Oncidium hybridum

  • LI Li ,
  • LUO Jun-qin ,
  • XU Jian-guo ,
  • NIE Zhen-peng ,
  • KE Fu-zhi ,
  • SUN Jian-hua
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  • Citrus Research Institute of Zhejiang Province, Huangyan 318020, Zhejiang China

Received date: 2009-06-01

  Online published: 2009-09-10

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Abstract

The effects of minimal medium,categories and concentration of plant hormone on the induction,proliferation and rooting cultivation of multiple shoots of stem segments in pedicel of Oncidium hybridum were studied by orthogonal experiment.The results showed that 1/2 MS medium was the best minimal medium for the growth of multiple shoots,the best induction medium was 1/2 MS+6-BA 5.0 mg/L+NAA 0.5 mg/L+sugar 20 g/L,the best proliferation medium was 1/2 MS+6-BA 3.0 mg/L+NAA 0.5 mg/L+sugar 30 g/L,and the best rooting cultivation medium was 1/2 MS+IBA 0.05 mg/L+NAA 0.3 mg/L+banana slurry 100 g/L+sugar 20 g/L.

Key words： Oncidium hybridum; orthogonal experiment; stem segments in pedicel; plantlet regeneration

Cite this article

LI Li , LUO Jun-qin , XU Jian-guo , NIE Zhen-peng , KE Fu-zhi , SUN Jian-hua . Studies on Influencing Factors of Plantlet Regeneration of Stem Segments in Pedicel of Oncidium hybridum[J]. Subtropical Plant Science, 2009 , 38(03) : 21 -23 . DOI: 10.3969/j.issn.1009-7791.2009.03.005

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