The mixed substrate consisting of orchard soil,common substrate for flower and wormpost which were mixed by volume ratio of 6:3:1,could significantly improve the survival rate and the robust level of the seedlings of Emilia sonchifolia and shorten its time to seedlings.The growth and the yield of E. sonchifolia were affected by the thickness of substrate,light intensity and spacing in the rows in varying degree.The root growth of E. sonchifolia was mainly affected by light intensity and the thickness of substrate,while with adequate light and thick substrate,its root system was stronger.The stem growth of E. sonchifolia was mainly affected by light intensity and secondly by spacing in the rows.But the leaf growth was less affected by any one of the three ecological factors.The yield of E. sonchifolia was most affected by spacing in the rows and was less affected by light intensity and the thickness of substrate.It showed that the reasonable close planting could improve the yield.On the other hand,appropriate shading raised the total flavonoids content of E. sonchifolia,but extended the time for squaring.According to the studies,substrate thickness 7 cm,light intensity 50% and spacing in the rows 5 cm×25 cm gave the best results for cultivation of E. sonchifolia.