Subtropical Plant Science >

2009 , Vol. 38 >Issue 04: 1 - 4

DOI: https://doi.org/10.3969/j.issn.1009-7791.2009.04.001

Expression and Antibody Preparation of Sugarcane Mosaic Virus P1 Protein

  • GUO Ying ,
  • RUAN Miao-hong ,
  • LIU Jia ,
  • YANG Chuan-yu ,
  • ZHANG Mu-qing
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  • 1. Fujian Institute of Subtropical Botany, Xiamen 361006, Fujian China;
    2. Key Lab of Eco-physiology & Genetic Improvement for Sugarcane, Ministry of Agriculture, Fuzhou 350002, Fujian China

Received date: 2009-08-03

  Online published: 2009-11-10

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Abstract

The target gene was subcloned into the prokaryotic expression vector pET-32a,and transformed into E. coli Bl21(DE3),to obtain recombinant pET-32a-P1.Ultrasound results showed that the fusion protein existed in soluble form.Expression product had the same size with the target protein detected by SDS-PAGE.The antibody against the P1 protein was gained by immunized rabbit with target protein cut from SDS-PAGE.Indirect ELISA measurements proved that the antibody specificity was fine,because of that the dilution of 51200-fold not merely kept positive still,but also passed western blot.

Key words： Sugarcane mosaic virus; P1; fusion protein expression; antibody

Cite this article

GUO Ying , RUAN Miao-hong , LIU Jia , YANG Chuan-yu , ZHANG Mu-qing . Expression and Antibody Preparation of Sugarcane Mosaic Virus P1 Protein[J]. Subtropical Plant Science, 2009 , 38(04) : 1 -4 . DOI: 10.3969/j.issn.1009-7791.2009.04.001

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