Subtropical Plant Science >

2009 , Vol. 38 >Issue 04: 27 - 30

DOI: https://doi.org/10.3969/j.issn.1009-7791.2009.04.008

Optimization for SRAP-PCR System of Clausena lansium Based on Orthogonal Design

  • YANG Xiang-hui ,
  • HUANG Jian-feng ,
  • FU Jia-xin ,
  • XIE Jiang-hui
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  • 1. College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong China;
    2. South Subtropical Crops Research Institute, CATAS, Zhanjiang 524091, Guangdong China

Received date: 2009-09-23

  Online published: 2009-11-10

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Abstract

The orthogonal design was used to optimize SRAP-PCR amplification system on Clausena lansium on four levels of five factors (Taq polymerase,Mg2+,DNA template,dNTPs,and primer,respectively).The results indicated that the order of each factor in different levels affected on the result of PCR was Mg2+ or Taq polymerase,DNA template,primer and dNTPs.An optimal SRAP-PCR system was established,that was total 25 μl reaction system containing PCR10×Buffer 2.5 μl,0.75U DNA polymerase,2.0 mmol/L Mg2+,60 ng DNA template,0.2 mmol/L dNTPs,and 0.2 μmol/L primer.

Key words： Clausena lansium; SRAP; orthogonal design; optimization

Cite this article

YANG Xiang-hui , HUANG Jian-feng , FU Jia-xin , XIE Jiang-hui . Optimization for SRAP-PCR System of Clausena lansium Based on Orthogonal Design[J]. Subtropical Plant Science, 2009 , 38(04) : 27 -30 . DOI: 10.3969/j.issn.1009-7791.2009.04.008

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