The effects of kanamycin with different concentrations and different treatment times on seed germination and seedlings growth of Chinese Zoysia were studied by indoor germination experiment.The results indicated that the difference in seed germination between treatments with kanamycin and without kanamycin was not significant,and the differences of different concentrations and different treatment times on the growth of seedlings were significant.With the increasing of kanamycin concentration,the plant height,root growth and lateral root growth of Zoysia were inhibited,and albinism rate increased at the same time;With the prolonging of treatment time,plant height and root growth decreased.According to comprehensive index,75 mg/L kanamycin treated for 6~10 d gave the best result for the growth of Zoysia sinica seedlings.