Subtropical Plant Science >

2009 , Vol. 38 >Issue 04: 62 - 65

DOI: https://doi.org/10.3969/j.issn.1009-7791.2009.04.017

Study of Tissue Culture Technology in Dharma Orchid

  • HE Guan-rong ,
  • HE Bi-zhu
Expand
  • 1. College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China;
    2. Horticulture College, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China

Received date: 2009-09-27

  Online published: 2009-11-10

Fold

Abstract

The trial used the original seedling stems of dharma orchid for tissue culture technology study,selected out the optimum medium:(1) protocorm induced:MS+6-BA 0.5 mg/L+NAA 1.0 mg/L+banana 250 g/L;(2) bud induced:1/2 MS+6-BA 3 mg/L+NAA 2.0 mg/L+AC 2.5 mg/L+su 3%+banana 200 g/L;(3) shoot proliferation:1/2 MS+6-BA 3 mg/L+NAA 2.0 mg/L+AC 2.5 mg/L+su 3%+banana 150 g/L;(4) rooting:1/2 MS+6-BA 0.3 mg/L+IBA 0.5 mg/L+NAA 0.5 mg/L+AC 2.5 mg/L+su 3%.Using mixture of perlite,fine stone and bark as culture substratum,the survival rate was up to 95%.

Key words： dharma orchid; stem; tissue culture; explant

Cite this article

HE Guan-rong , HE Bi-zhu . Study of Tissue Culture Technology in Dharma Orchid[J]. Subtropical Plant Science, 2009 , 38(04) : 62 -65 . DOI: 10.3969/j.issn.1009-7791.2009.04.017

Options
Outlines

/

〈 〉

Copyright © Editorial office of Subtropical Plant Science
Tel: 0592-5654157　E-mail: yrdzwkx@126.com
Supported by Beijing Magtech Co., Ltd.