The ZDS gene was cloned from total RNA of flower in Narcissus tazetta var. chinensis by RACE technology. The expression analysis of ZDS gene was predicted with some related software tools, and the expression of ZDS gene in different floral organ of different varieties was analysis with real-time PCR. The sequence analysis indicated that the length of ZDS is 2189 bp, it contains an ORF encoding 499 amino acid residues(Mw=63.6 kDa), as well as 5' non-coding region with 69 bp and 3' non-coding region with 395 bp, named as Ntzds (GenBank accession number EU573238). Compared with ZDSs of other plants, Ntzds amino acid sequence has 97%, 85%, 83% identity with that of Narcissus pseudonarcissus, Vitis vinifera and Citrus unshiu respectively. Expressions of Ntzds in petals and corona from N. tazetta var. chinensis, ‘Minnow’, ‘Fortissimo’ were investigated by real-time qPCR. The results showed that Ntzds gene expression was different in three varieties. The expression level in corona was higher than in petals in same variety; we concluded that the transcription level of Ntzds was developing with the deepening of flower color.
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