The tender stem of Anthurium andraeanum was used as explants to study the effects of different growth regulators concentrations on the stem bud's germination, proliferation, rooting culture and the prevention of callus during proliferation culture. The results indicated that the optimal culture medium for stem bud inducement was MS + 6-BA 2.0 mg/L + NAA 0.5 mg/L, with the germination rate was 87.5%；the optimal culture medium for proliferation was MS + 6-BA0.8 mg/L + NAA 0.2 mg/L + VB2 8.0 mg/L, with the multiplication coefficient was 3.8; the optimal culture medium for rooting was 1/2MS + NAA0.5 mg/L, with the rooting rate was 98%; in addition, appropriate VB2 in proliferation medium could efficaciously prevent the form and differentiation of callus, thus reaching the goal that propagating buds by buds.