In this study, dopamine was polymerized onto the surface of optically transparent ITO (or Au) electrode by auto-oxidation method. The formed polydopamine film was then protonated in pH 3 buffer solution to become positively charged which facilitates electrostatic interaction with negatively charged protoplasts. The effectiveness of the protonated polydopamine films to the adhesion of Arabidopsis thaliana protoplasts was confirmed by cyclic voltammetry and electrochemical impedance spectroscopy. In the tested range of 1000—30 000 protoplasts, the charge-transfer resistance (Rct) of the protonated polydopamine films increased with the protoplast number Ncells, and the 1/Rct displayed a linear relationship with 1/Ncells. Moreover, the dynamic adhesion test results using quartz crystal microbalance further proved that the protonated polydopamine films had good adhesion to the protoplasts. This study provides an effective method for the immobilization and sensing of protoplasts, which is an important step toward the goal of study of plant structure, function and behavior at the cellular level and the study of plant life diversity.
ZHANG
,
NA
,
DENG
,
JUN
,
XIE
,
JIE
,
ZHOU Tie-An
,
SU Zhao-Hong
,
SHEN Da-Zhong
,
CHEN Zong-Xing
,
ZOU Jian-Feng
. Adhesion and Detection of Arabidopsis Protoplasts Based on Surface Protonated Poly(dopamine) Modified Electrode[J]. Subtropical Plant Science, 2017
, 46(02)
: 101
-107
.
DOI: 10.3969/j.issn.1009-7791.2017.02.001
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