With different polarity organic solvents (n-hexane, dichloromethane, ethyl acetate, acetone and ethanol), extraction yield of Voacanga africana seeds by Soxhlet extraction were determinated. The inhibitory activity of extracts against human tumor cell proliferation was tested using Hela Cells and SMMC-7721 Cells as the experimental object and the MTT method as judge standards. The results indicated that V. africana seed extracts of acetone showed excellent inhibitory activity on the proliferation of SMMC-7721 cells and a general inhibition towards Hela cancer cells. And the n-hexane extracts also had relatively significant cytotoxic activity on Hela cancer cells, while the activity towards SMMC-7721 cells was not significant; other organic phase extracts possessed almost no inhibition activity.
ZHOU
,
JING
,
LIU Jia-Jia
,
WU Yi-Qiang
,
YUAN Guang-Ming
. Antitumor Activity of Extracts from Voacanga africana Seeds[J]. Subtropical Plant Science, 2017
, 46(02)
: 113
-116
.
DOI: 10.3969/j.issn.1009-7791.2017.02.003
[1] 康炼常,林德钦,郑志忠,明艳林,童庆宣. 非洲马铃果化学成分及其生物活性研究进展[J]. 中药材, 2012,35(11): 1877—1881.
[2] Tan P V, Penlap V B, Nyasse B, Nguemo J D B. Anti-ulcer actions of the bark methanol extract of Voacanga africana in different experimental ulcer models in rats[J]. Journal of Ethnopharmacology, 2000,73(3): 423—428.
[3] Olaleye S B, Oke J M, Etu A K, Omotosho I O, Elegbe R A. Antioxidant and anti-inflammatory properties of a flavonoid fraction from the leaves of Voacanga africana[J]. Nigerian Journal of Physiological Sciences Official Publication of the Physiological Society of Nigeria, 2004(1–2): 69.
[4] La B J, Gillo L. Cardiotonic properties of a new alkaloid extract from Voacanga africana: voacaline[J]. Comptes Rendus Des Séances De La Société De Biologie Et De Ses Filiales, 1956,150(8–9): 1628—1630.
[5] St?ckigt J, Pawelka K H, Tanahashi T, Danieli B,Hull W E. Voafrine A and Voafrine B, new dimeric indole alkaloids from cell suspension cultures of Voacanga africana stapf[J]. Helvetica Chimica Acta, 1984,66(8): 2525—2533.
[6] Liu X, Yang D, Liu J, Ren N. Analysis of essential oils from Voacanga africana seeds at different hydrodistillation extraction stages: chemical composition, antioxidant activity and antimicrobial activity[J]. Natural Product Research, 2015,29(20): 1950—1953.
[7] 郑志忠,陈良华,郑国华,童宇星,张树峰,童庆宣,明艳林. 七种中草药提取物抗肿瘤活性部位的筛选研究初报[J]. 亚热带植物科学, 2011,40(3): 31—35.
[8] Beekman A C, Woerdenbag H J, Kampinga H H, Konings W T. Cytotoxicity of artemisinin, a dimer of dihydroartemisinin, artemisitene and eupatoriopicrin as evaluated by the MTT and clonogenic assay[J]. Phytotherapy Research, 2015,10(2): 140—144.
[9] Sebaugh J L. Guidelines for accurate EC50/IC50 estimation[J]. Pharmaceutical Statistics, 2011,10(2): 128—134.
[10] 李纲,陶莉,王洪林,陈剑,左丽,杨梅. Ⅲ期非小细胞肺癌多学科综合治疗的临床效果[J]. 现代肿瘤医学, 2010,18(6): 1117—1119.
[11] 张璐璐. 大蒜素对丙烯酰胺所致小鼠组织氧化损伤的保护机制研究[D]. 长春: 吉林大学硕士学位论文, 2013.
