Primary culture, subculture, rooting culture and transplantation of Asplenium nidus spores were carried out in this study. Results showed that the best primary culture medium was MS＋0.1% activated carbon, the formation rate of prothallus was 80%; In the multiplication medium with 1/3MS ＋6-BA 1.8 mg·L-1＋NAA 0.5 mg·L-1, the proliferation times was up to 5.1; in rooting medium with 1/2MS＋NAA 0.5 mg·L-1＋IBA 0.5 mg·L-1, the rooting rate of subculture shoot was highest; the best transplanting substrate was the red soil＋humus (1∶1), transplanting survival rate was 90%.
WANG Chang-Shui
. Cultivation Techniques of Tissue Culture Container Seedlings of Asplenium nidus[J]. Subtropical Plant Science, 2017
, 46(02)
: 192
-194
.
DOI: 10.3969/j.issn.1009-7791.2017.02.020
[1] 李文安,王玉琴. 狼尾蕨的离体培养[J]. 植物生理学通讯,1993,29(3): 44.
[2] 尹怀约. 贯众叶片愈伤组织的诱导和植株再生[J]. 植物生理学通讯, 1989(4): 39—45.
[3] 郑若仙. 彩叶凤尾蕨孢子离体繁殖与组织培养[J]. 植物杂志, 1992,28(2) : 8.
[4] 金建平,兰涛. 皱叶肾蕨卷曲叶尖的离体培养[J]. 植物生理学通讯, 1992,28(5) : 359—360.
[5] 黄韶玲,张洁莲. 鹿角蕨的组织培养[J]. 植物生理学通讯, 1993,29(1): 25.
[6] 彭晓明,曾宋君. 铁线蕨的组织培养及植株再生[J]. 植物生理学通讯, 2004,40 (5) : 575.
[7] 秦廷豪,邹宗兰. 鸟巢蕨的组织培养[J]. 植物生理学通讯, 2004,40(3): 349.
[8] 杨广瑜. 鸟巢蕨植物组织培养工厂化育苗技术[J]. 农业工程技术(温室园艺), 2006(8): 58—59.
[9] 陈金典. 鸟巢蕨组织培养育苗技术研究[J]. 福建农业科技, 2010(2): 44—46.
[10] 洪伟. 林业试验设计技术与方法[M]. 北京: 北京科学技术出版社, 1993．
