Taking 174 individuals of Tetracentron sinense 26 populations as materials, 3 sampling strategies (random sampling strategy, allele preferred sampling strategy and deviation sampling strategy) were used to construct its core collection based on ISSR markers. Genetic diversity indexes were used to determine the most suiconstruction method. 180 bands were amplified14 ISSR primers, and the observed number of alleles(Na), effective number of alleles(Ne), Nei’s genetic diversity(H) and Shannon information index(I) of original germplasm were 1.6944, 1.3912, 0.2321, 0.3509 respectively, indicating the abundant genetic diversity of T. sinense was low. Genetic diversity indexes of the core collection constructedallele preferred sampling strategy were higher than those of other two strategies. 78 core collection resources of T. sinenseallele preferred sampling strategy accounted for 44.83% initial collection, and the retention rate of genetic diversity indexes(Na, Ne, H and I) were respectively 98.69%, 100.01%, 99.27%, 98.67%, which indicated that the genetic diversity and variation were well preserved. The result demonstrated that the allele preferred sampling strategy was the most appropriate method for core collection construction of T. sinense.
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