The optimum process of ultrasound-assisted extraction of total polyphenos from Moringa oleifera leaves by response surface methodology was studied in the research. The experiment method was designed according to Box-Behnken central composite design with 4 factors and 3 levels on the basis of single-factor experiment of ethanol concentration, material-to-liquid ration, extraction time and extraction times. The relationship between influencing factors and response values was subsequently established. The results showed that the optimum extraction conditions were as follows: the extracting solvent was 62% ethanol, material to liquid ration was 1:30 (W/V), extracting time was 30 min, and extracting three times. Under these conditions, the maximal yield of total polyphenols was upto 27.32 mg·g-1, which was well matched with the predicted content.
FU Wen-qun
,
SUN Yan-yan
,
LI Xiao-hua
,
SHAO Nan-jin
,
HUANG Gui-hong
,
SU Xue-yan
,
ZHENG Yi-mei
. Optimization of Ultrasound-assisted Extraction of Total Polyphenols from Moringa oleifera Leaves by Box-Behnken Response Surface Methodology[J]. Subtropical Plant Science, 2018
, 47(02)
: 107
-112
.
DOI: 10.3969/j.issn.1009-7791.2018.02.002
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