Plant physiology, biochemistry and molecular biology

Root Development in Arabidopsis Folylpolyglutamate Synthetase Mutant adtfb under Low-nitrogen Condition

  • MENG Hong-yan ,
  • WANG Wen-hua ,
  • ZHANG Chun-yi
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  • (1.Fujian Institute of Subtropical Botany/Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Xiamen 361006, Fujian China; 2.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

Received date: 2018-07-23

  Revised date: 2018-08-06

  Online published: 2018-09-30

Abstract

T-DNA insertion mutant of folylpolyglutamate synthetase atdfb was used to explore the role of folates during root development in Arabidopsis. Phenotype of three-week-old atdfb seedlings was similar to the wild type, however, the mutant displayed shorter primary root than the wild type on 1/2MS, and phenotype of complemented seedling was recovered indicating that the shorten root phenotype was due to the loss function of AtDFB. Length of primary root of 11-day-old atdfb-3 seedlings was 23% of the wild type on 1/2MS medium that was nitrogen (N)-sufficient conditions. The development of atdfb-3 almost stagnated under low-N conditions, and the primary root length of 11-day-old atdfb-3 seedlings was only 4% of the wild type. Under low-N conditions, exogenous 5-formyl-tetrahydrofolate (5-F-THF) restored the phenotype of atdfb-3 to the wild type. With the application of 5-F-THF, the primary root length, root hair length and the quiescent center cell organization in atdfb-3 were recovered. This study indicated the necessity of folates in Arabidopsis root development and adapting to low-N condition.

Cite this article

MENG Hong-yan , WANG Wen-hua , ZHANG Chun-yi . Root Development in Arabidopsis Folylpolyglutamate Synthetase Mutant adtfb under Low-nitrogen Condition[J]. Subtropical Plant Science, 2018 , 47(03) : 199 -206 . DOI: 10.3969/j.issn.1009-7791.2018.03.001

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