A simple and efficient system of plant DNA extraction and PCR amplification was established. The system only need one step to extract DNA, and good amplification results of target genes could be obtained by prolongation PCR predenaturation time. The system had strong experimental stability and universal species applicability. It would greatly improve the efficiency of plant molecular biology experiment and plant breeding experiment based on molecular marker, and decrease scientific research cost.
XIAO Shuai
,
LI Lin-xia
,
LI Hai-ou
. An Efficient Plant DNA Extraction and PCR Amplification System[J]. Subtropical Plant Science, 2018
, 47(03)
: 207
-210
.
DOI: 10.3969/j.issn.1009-7791.2018.03.002
[1] Doyle J J, Doyle J L. A rapid DNA isolation method for small quantities of fresh tissues[J]. Phytochemcal Bulletin, 1987,19: 11—15.
[2] Rogers H J, Burns N A, Parkes H C. Comparison of small—scale methods for the rapid extraction of plant DNA suitable for PCR analysis[J]. Plant Molecular Biology Reporter, 1996,14(2): 170—183.
[3] Rogers S O, Bendich A J. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues[J]. Plant Molecular Biology, 1985,5(2): 69—76.
[4] Jr C N S. Rapid DNA Extraction from Plants[M]. Berlin: Springer Berlin Heidelberg, 1997: 25—28.
[5] 韩玉杰,贾炜珑,王自霞,周小梅. 几种提取植物DNA方法的比较[J]. 山西农业科学, 2008,36(7): 17—19.
[6] 李金璐,王硕,于婧,周世良. 一种改良的植物DNA提取方法[J]. 植物学报, 2013,48(1): 72—78.
[7] 徐平丽,赵晋平,孟静静,李保龙,李新国,郭峰. 一种适宜拟南芥PCR检测的DNA提取方法[J]. 安徽农业科学, 2010,38(13): 6653—6654.
[8] 张志忠,吴菁华,吕柳新,陈桂信. 一种适于PCR检测的DNA微量提取方法[J]. 亚热带植物科学, 2004,33(1): 29—30.
[9] Bellstedt D U, Pirie M D, Visser J C, de Villiers M J, Gehrke B. A rapid and inexpensive method for the direct PCR amplification of DNA from plants[J]. American Journal of Botany, 2010, 97(7): e65.
[10] Hyunsik H, Shinchul B, Seungbum L, Yeonhee L, Chang A C. A rapid and simple genotyping method for various plants by direct-PCR[J]. Plant Breeding & Biotechnology, 2013,1(3): 290—297.
[11] Hosaka K. An easy, rapid, and inexpensive DNA extraction method, “one-minute DNA extraction,” for PCR in potato[J]. American Journal of Potato Research, 2004,81(1): 17—19.
[12] 戴剑,洪德林,张大栋,吴燕. 一种快速高效的DNA提取方法研究[J]. 麦类作物学报, 2011,31(3): 437—442.
[13] 孙林静,马忠友,苏京平,刘学军,王春敏,王胜军,闫双勇. 一种简单快速的DNA提取方法在水稻上的应用[J]. 天津农学院学报, 2007,14(3): 1—4.
[14] 汤文开,谭新,张辉,黄耿青,徐文亮,李学宝. 一种快速简单高效提取植物DNA的方法[J]. 华中师范大学学报(自科版), ss2007,41(3): 447—449.
[15] 黄萱,高丽美,张永彦,徐子勤. 一种优化的植物总DNA提取方法[J]. 西北植物学报, 2004,24(6): 1103—1106.
[16] 孙璐宏,鲁周民,张丽. 植物基因组DNA提取与纯化研究进展[J]. 西北林学院学报, 2010,25(6): 102—106.
