To investigate the anti-tumor activities of four indole alkaloids, Vincamine, Coronaridine, Voacamine and Voacangine in Voacanga africana in vitro, the proliferation inhibition effects of SKOV3, BEL7402, SMMC7721 and Changliver cells were determined by MTT assay, and the morphological changes of apoptosis were observed by AO/EB staining. The results of MTT assay showed that there was a dose dependent relationship between the proliferation inhibition of four cell lines and the four indole alkaloids. The growth inhibition rates of 50 μg·mL-1 Voacamine on all four cell lines were all over 95%; at the same concentration, Voacangine only inhibited BEL7402 and Changliver by more than 78%; Coronaridine had a proliferation inhibition rate of only 50% for Changliver; the effect of Vincamine on the proliferation of four cell lines was not obvious. After staining by AO/EB method, the four cell lines showed nuclear shrinkage, concentration and migration under the action of 12.5 μg·mL-1 Voacamine, indicating that Voacamine had obvious effect of inducing apoptosis; Voacangine only had the same effect on BEL7402 and Changliver, and the other two alkaloids acting cell lines showed no obvious apoptosis. The MTT and the AO/EB staining method showed consistent results. Voacamine and Voacengine could induce apoptosis of ovarian cancer cells and human hepatocytes, and anti-tumor effects of the other two alkaloids were relatively weak.
BAI Ya-zhu
,
ZHENG Zhi-zhong
,
CHEN Qing-zhi
,
HUANG Wen
,
CHEN Liang-hua
,
LIN He-tong
,
MING Yan-lin
. Inhibition Effects of Four Indole Alkaloids in Voacanga africana on Tumor Cells in vitro[J]. Subtropical Plant Science, 2019
, 48(03)
: 209
-214
.
DOI: 10.3969/j.issn.1009-7791.2019.03.001
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