A high-performance liquid chromatography method for determination of resveratrol was developed. The determination of resveratrol was performed on a Hypersil C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol-water (72:28), and the flow rate was 1 mL·min-1. The detector was photodiode array detector and the variable wavelength detector was set at 306 nm. In the above chromatographic conditions, good linear was observed in the linear range of 10～200 μg·mL-1, and the correlation coefficient (r) was 0.9999. The relative standard deviation (RSD) of the precision, reproducibility and stability were 0.77%, 0.41% and 0.21%, respectively. The average recovery of standard addition was 99.92% with RSD of 0.39%. The method is sensitive, reliable, accurate, and could be used for the determination of resveratrol.
HE En-ming
,
TIAN Miao
,
SHEN Rui-chi
,
REN Yu-jun
,
WANG Wen-hua
. Determination of Resveratrol in Vitis thunbergii var. taiwaniana Leaves by HPLC[J]. Subtropical Plant Science, 2019
, 48(04)
: 334
-338
.
DOI: 10.3969/j.issn.1009-7791.2019.04.005
[1] 张铭文. 细本山葡萄栽培技术[J]. 苗栗区农业专讯, 2009(45): 4—8.
[2] 胥俊越,陈全成,林挺,孙翠玲,郑旗志,徐士兰,陈海峰. 小叶山葡萄的化学成分研究[J]. 中草药, 2013,44(6): 660—664.
[3] Hwang D, Lim Y H. Resveratrol antibacterial activity against Escherichia coli is mediated by Z-ring formation inhibition via suppression of FtsZ expression[J]. Scientific Reports, 2015(5): 10029—10038.
[4] Rafe T, Shawon P A, Salem L, Chowdhury N I, Kabir F, Zahur S M B, Akhter R, Noor H B, Mohib M M, Sagor M A T. Preventive role of resveratrol against inflammatory cytokines and related diseases[J]. Current Pharmaceutical Design, 2019,25(12): 1345—1371.
[5] Fernández-Castillejo S, Macià A, Motilva M J, úrsula C, Rosa S. Endothelial cells deconjugate resveratrol metabolites to free resveratrol: a possible role in tissue factor modulation[J]. Molecular Nutrition & Food Research, 2019,63(3): 1800715—1800726.
[6] Sun J, Zhang M, Chen K, Chen B D, Zhao Y Y, Gong H, Zhao X, Qi R M. Suppression of TLR4 activation by resveratrol is associated with STAT3 and Akt inhibition in oxidized low-density lipoprotein-activated platelets[J]. European Journal of Pharmacology, 2018,836: 1—10.
[7] 于飞,李岩溪,任亚浩,王博涵,郑晓楠,康思雯,赵越,杨军. 白藜芦醇对高脂模型小鼠脂代谢影响[J]. 中国公共卫生, 2010,26(11): 1401—1402.
[8] Gresele P, Cerletti C, Guglielmini G, Pignatelli P, Gaetano G D, Violi F. Effects of resveratrol and other wine polyphenols on vascular function: an update[J]. The Journal of Nutritional Biochemistry, 2011,22(3): 201—211.
[9] Dominique B R. Resveratrol and cardiovascular diseases[J]. Nutrients, 2016,8(5): 250—273.
[10] Bhat K P L, Pezzuto J M . Cancer chemopreventive activity of resveratrol[J]. Annals of the New York Academy of Sciences, 2002,957(1): 210—229.
[11] Ca D L L, Villegas I. Resveratrol as an anti-inflammatory and anti-aging agent: mechanisms and clinical implications[J]. Molecular Nutrition & Food Research, 2005,49(5): 405—430.
[12] Jin F, Wu Q, Lu Y F, Lu Y F, Gong Q H, Shi J S. Neuroprotective effect of resveratrol on 6-OHDA-induced Parkinson’s disease in rats[J]. European Journal of Pharmacology, 2008,600(1): 78—82.
[13] Karuppagounder S S, Pinto J T, Xu H, Chen H L, Beal M F, Gibson G E. Dietary supplementation with resveratrol reduces plaque pathology in a transgenic model of Alzheimer’s disease[J]. Neurochemistry International, 2009,54(2): 111—118.
[14] Li F, Gong Q, Dong H, Shi J. Resveratrol, a neuroprotective supplement for Alzheimer’s disease[J]. Current Pharmaceutical Design, 2012,18(1): 27—33.
[15] 贾文斌,钱跹,吕磊. 薄层色谱法和高效液相色谱法在白藜芦醇胶囊定性定量研究中的应用[J]. 海军医学杂志, 2017,38(6): 506—508.
[16] 许士明,李林林,徐正,邓宁,王恬. 葡萄酒中白藜芦醇2种检测方法对照及存在问题分析[J]. 中国卫生检验杂志, 2018,28(20): 2553—2555.
[17] 钟永红,苏流坤,刘敏,宋玉梅,黄子荣,梁家佑,温才洁. 高效液相色谱法测定葡萄制品中的白藜芦醇[J]. 山东化工, 2019,48(7): 99—100, 102.
