Response surface methodology was used to optimize the extraction technology of betulinic acid from Marsdenia tenacissima. Based on single-factor experiments, ultrasonic power, extraction duration, material-liquid ratio, and times of extraction were selected as influencing factors during extraction. The experiment mathematical model was arranged according to Box-Behnken central composite experiment design, which studied each variable and their interactions on the extraction yield of betulinic acid. The quadratic multinomial model on the extraction yield of betulinic acid and each influence factor of extraction betulinic acid from M. tenacissima was established by using the Design-Expert software. And the optimum conditions were as follows: ultrasonic power 150 W, material-liquid ratio 1:25, extraction duration 20 min and extraction twice. The predicted value of extraction yield of betulinic acid was 0.314% under the optimum conditions, and the experimental value was 0.311%.
SHI Ling
,
WANG Ze-feng
,
ZHANG Wei
,
MIN Yong
. Extraction Optimization of Betulinic Acid from Marsdenia tenacissima Using Response Surface Methodology[J]. Subtropical Plant Science, 2020
, 49(02)
: 93
-98
.
DOI: 10.3969/j.issn.1009-7791.2020.02.002
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