Plant molecular biology

Genetic Diversity Analysis of Camellia sinensis in Dongshan, Suzhou by ISSR

  • LI Hao-yu ,
  • YANG Da-qiang ,
  • WANG Li-fen ,
  • ZHANG Zhong-xin ,
  • ZHENG Jun-hua ,
  • HAN Zhao-lan ,
  • ZHU Xu-jun ,
  • FANG Wan-ping ,
  • YIN Jiang-hai
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  • (1.Wuzhong Dongshan Agricultural and Forestry Service Station, Suzhou 215107, Jiangsu China; 2.Gold Mantis School of Architecture, Suzhou University, Suzhou 215325, Jiangsu China; 3.Tea Research Institute, Nanjing Agricultural University, Nanjing 210095, Jiangsu China; 4.Suzhou Polytechnic Institute of Agriculture, Suzhou 216000, Jiangsu China)

Received date: 2020-02-23

  Revised date: 2020-04-02

  Online published: 2020-06-30

Abstract

The genetic diversity of tea plant in Dongting district of Suzhou was examined by ISSR. The results showed that 76 bands were amplified with 6 ISSR primers, the polymorphic bands of which were 70, and the polymorphism percentage was 92.11%. The genetic similarity was from 0.37-0.89. The GS was the greatest between Baisha and No.1 Dongling, and they had the highest genetic similarity and the smallest genetic distance. Cluster analysis classified the 51 cultivars into 2 major groups by UPGMA. Each group was divided into four subgroups and the genetic relationship was closer in the same subgroups. Among them, some different strains from the same breed had higher genetic similarity coefficients. The results suggested that ISSR was a useful tool for molecular genetic diversity and relationship analysis of tea cultivars.

Cite this article

LI Hao-yu , YANG Da-qiang , WANG Li-fen , ZHANG Zhong-xin , ZHENG Jun-hua , HAN Zhao-lan , ZHU Xu-jun , FANG Wan-ping , YIN Jiang-hai . Genetic Diversity Analysis of Camellia sinensis in Dongshan, Suzhou by ISSR[J]. Subtropical Plant Science, 2020 , 49(03) : 163 -167 . DOI: 10.3969/j.issn.1009-7791.2020.03.001

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