To investigate the deproteinization process of polysaccharides extracted from Smilax glabra Roxb. by response surface methodology. Polysaccharide was extracted by water?extraction and alcohol?precipitation.?And a sevage method was used in the deproteinization progress. Through the detection of deproteinization efficiency and polysaccharide retention rate, the deproteinization process of polysaccharides extracted from S. glabra Roxb. was optimized by response surface methodology. The results showed that the best deproteinization conditions were as follows: sevage reagent:the test liquid=1:3; shaking intensity, 8 (range1-10); shaking time, 10 min; and deprieinization times, 5; giving a percentage of deproteinization of 97.39% and polysaccharide retention of 74.13%. The optimized process had good repeatability and small error, and could be used in the process of S. glabra Roxb. polysaccharide deproteinization.
LI Cong
,
QIU Kun-cheng
,
XIE Zhi-kuan
,
XIE Yu-bin
,
ZHU Wei
. Optimization of Deproteinization Process of Polysaccharides Extracted from Smilax glabra Roxb. by Response Surface Methodology[J]. Subtropical Plant Science, 2020
, 49(04)
: 264
-269
.
DOI: 10.3969/j.issn.1009-7791.2020.04.004
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