In this study, the genetic transformation system of jasmine was studied. The stem segment of the annual shoots of Jasminum sambac was used as the explants. The results showed that the resistant stem segments and callus with high survival rate could be obtained under the conditions that treating with agrobacterium infection after 7 d pre-culture, and then culturing on the screening medium with Kan concentration of 80 to 100 mg·L-1. The GFP gene was transformed into jasmine stem segments and screened, and GFP fluorescence was detected in the stem segments that pre-cultured for 7 d and then screened for 3 d or 30 d after transformation, and also detected in the stem callus that pre-cultured for 20 d and screened for 14 d after transformation, indicating that GFP gene successfully transformed into jasmine stem segments. The results indicated that the transformation system was feasible, which laid a foundation for subsequent functional studies of jasmine genes.
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