Sulfuric acid phenol method, AlCl3 method and acid dye colorimetry were used to determine the contents of polysaccharides, flavonoids and alkaloids in flowers, leaves and stems of Dendrobium officinale. DPPH and ABTS clearance test were used to evaluate the antioxidant activities in vitro of water extracts and ethanol extracts from flowers, leaves and stems of D. officinale. The results showed that the content of polysaccharide: stem>flower>leaf, flavonoid content: flower>leaf>stem. Alkaloids were less distributed in all parts. The content of flavonoids in flower was 1.847%, the content of polysaccharide in stem was 23.92%. The DPPH radical scavenging capacity was as follows: flower water extract, stem alcohol extract, flower alcohol extract, the half effect concentration (EC50) were 410.4 μg·mL-1, 454.1 μg·mL-1, 573.2 μg·mL-1, respectively. The ABTS radical scavenging ability was as follows: flower alcohol extract, flower alcohol extract, flower water extract, the half effect concentration (EC50) were 61.1 μg·mL-1, 62.2 μg·mL-1, 103.0 μg·mL-1, respectively. The antioxidant activity of D. officinale flower was better than that of leaves and stems, and the antioxidant capacity of ethanol extract was better than that of water extract.
GENG Wen-hui
,
LU Shao-ji
,
MING Yan-lin
. The Chemical Constituents and Antioxidant Activities of Dendrobium officinale[J]. Subtropical Plant Science, 2020
, 49(06)
: 438
-442
.
DOI: 10.3969/j.issn.1009-7791.2020.06.005
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