In the cuttings propagation experiment, the semi-lignified branches of 4-year-old Camellia oleifera ‘Cenruan 2’ were treated with different hormone types, the changes of peroxidase activity, soluble protein and chlorophyll in the cutting woods rooting process were studied, and the relationship between cutting woods and biochemical indices was discussed. The results showed that the activities of peroxidase, soluble protein and chlorophyll were significant difference in different days after softwood cutting (P<0.01). POD activities showed double peak curve of ‘rise, fall, rise sharply and down’, while soluble protein showed curve of ‘rise, fall, rise’, chlorophyll showed curve of ‘fall, rise, down and rise’. Compared to the control, the pear of curve of peroxidase, soluble protein and chlorophyll obviously increased. It was concluded that hormone directly affected the rooting ability of Camellia oleifera cuttings.