Kudingcha, which is rich in chlorogenic acid, was used as material, the extraction efficiency of chlorogenic acid isomers and changes of isomers after extraction were investigated by using methanol, ethanol, acetone and water, combined with the commonly used ultrasonic extraction, water bath extraction and reflux extraction. Six chlorogenic acid isomers and caffeic acid from Kudingcha could be separated within 6 minutes by ultra performance liquid chromatography (UPLC). The results showed that acetone used as extraction solvent could obtain higher extraction efficiency in ultrasonic and water bath, and it was easy to obtain more high content of total chlorogenic acids and isochlorogenic acid A. However, the most commonly used solvent ethanol could not perform the ideal extraction effect under the set conditions of ultrasonic wave and water bath extraction. In the reflux extraction of different solvents, although the total amount of chlorogenic acids extracted was similar, the contents of isochlorogenic acid A and isochlorogenic acid C showed great differences. The reflux extraction of alcohol solution, especially ethanol solution, greatly increased the amount of isochlorogenic acid C, and the corresponding amount of isochlorogenic acid A decreased significantly, which indicated that under the condition of alcohol heating, isochlorogenic acid A was converted into isochlorogenic acid C, which also provided a new and simple method for obtaining isochlorogenic acid C, the ingredient with stronger antioxidant capacity.
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