In this study, 27 new self-bred germplasms of Petunia hybrida were selected to study the flower color and their pigments. The flower color phenotype of P. hybrida was described by RHSCC and colorimeter, the pigment types in their petals were preliminarily determined by color reaction, and the content of three kinds of pigments in petals of selected P. hybrida was determined by standard curve method or pH differential method. The flower color of all germplasms could be classified into 5 series, and the color ranges mainly from purplish red to red. The flower color of P. hybrida was widely distributed in CIELab color system, and the color parameters were distinguished greatly in petunia petals with different color. The petals of P. hybrida contained flavonoids and anthocyanins, but no or little carotenoids. The flavonoid content in petals of 13 tested germplasms ranged from 2.5?to 12.2 mg·g–1 FWmg/g·FW, in which the anthocyanin content ranged from 0.03 to 3.88 mg·g–1 FWmg/g·FW. The carotenoid content in petunia petals was much lower than the content of flavonoids, the highest and the lowest value was present 0.216?mg·g–1 FWmg/g·FW and 0.004 mg·g–1 FWmg/g·FW, respectively. Taken together, the type and the content of pigments were different in the petunia petals within different color; no or little carotenoids were detected in petals of most petunia germplasms of purplish red and red series; a high level of flavonoids was observed in petals of yellow, white and purple petunias, and the content of anthocyanins in petals of purple and purplish red petunias was also relatively high.
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