Based on the single factor of experiment, the effects of temperature and IPTG concentration on BL21(DE3)/pProEXHTa-PIUGT2 protein production were studied. Fermentation conditions of BL21(DE3)/pProEXHTa-PIUGT2 cultured in 150 ml LB medium were optimized. The expression conditions of PlUGT2 protein was high when culture condition to be 0.75% of IPTG concentration and 20℃.
LI Jian-mei
,
LV Min
,
HU Xiao-xiang
,
LI Ling
. Prokaryotic Expression and Optimization of Glucosyltransferases PlUGT2 from Pueraria lobata (Willd.) Ohwi[J]. Subtropical Plant Science, 2012
, 41(02)
: 9
-12
.
DOI: 10.3969/j.issn.1009-7791.2012.02.003
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