Plant physiology, biochemistry and molecular biology

Identification and Expression Analysis of Trihelix Transcription Factor Family in Tobacco

  • 李栋成,李魁印,韦兴启,段丽丽,莫泽君,刘仁祥
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  • (1. Tobacco College of Guizhou University / Key Laboratory of Tobacco Quality Research of Guizhou Province, Guiyang 550025, Guizhou China; 2. Agricultural College of Anshun College, Anshun 561000, Guizhou China; 3. Changshun County Branch of Qiannan Tobacco Company, Changshun 550700, Guizhou China)

Received date: 2022-01-02

  Accepted date: 2022-01-25

  Online published: 2022-05-17

Abstract

Tobacco is a kind of temperature-loving economic crop. Screening and utilization of cold-resistant genes plays an important role in the growth and development, yield and quality of tobacco. In this study, bioinformatics analysis was used to identify and analyze the physical and chemical properties, chromosome distribution, chromosome collinearity, motif composition, gene structure, cis-acting elements and expression of Trihelix family genes in tobacco genome data, and the structure and function of tobacco Trihelix family members were preliminarily analyzed. Thirty-two Trihelix genes were identified from common tobacco, which were divided into 5 subfamilies: GT-1, GT-2, GTγ, SH4 and SIP1. Thirty-two NtTH genes had 10 different motifs and different gene structures, but were similar in the same subfamily. There were homologous Trihelix genes in tobacco, tomato, Arabidopsis and rice, and there were collinear genes in the Trihelix family of common tobacco. Thirteen different types of cis elements were identified, which were mainly related to abiotic stress, hormone and growth and development. ABRE and MeJA were the two most cis elements, indicating that the function of NtTH gene was mainly related to abiotic stress and the regulation of growth and development response. Among them, 11 genes, such as tobacco NtTH3 and NtTH16, played an important role in response to low temperature stress. In this study, the genes related to cold resistance of tobacco were found, which provided a theoretical basis for the genetic improvement of cold resistance and stress resistance of tobacco.

Cite this article

李栋成,李魁印,韦兴启,段丽丽,莫泽君,刘仁祥 . Identification and Expression Analysis of Trihelix Transcription Factor Family in Tobacco[J]. Subtropical Plant Science, 2022 , 51(1) : 1 -12 . DOI: 10.3969/j.issn.1009-7791.2022.01.001

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