Plant physiology, biochemistry and molecular biology

Identification and Analysis of Original Plants of Polygonati Rhizoma by UV Fingerprint Combined with Chemometrics

  • 杜泽飞,张 霞,温 翔,段宝忠
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  • (1. Pharmacy Department of Gejiu People’s Hospital, Honghe 661000, Yunnan China; 2. College of Pharmaceutical Science, Dali University, Dali 671000, Yunnan China)

Online published: 2022-08-03

Abstract

A method for rapid identification of different original plants of polygonati rhizoma was established by UV spectroscopy combined with chemometrics. The best extraction solvent, time and dosage of polygonati rhizoma were determined by single factor experiment, and the test solution was prepared. The UV fingerprint of three species of Polygonatum was established by UV spectroscopy. After the spectral data were transformed, principal component analysis (PCA) and hierarchical cluster analysis (HCA) were carried out. The method had good reproducibility, precision and stability. The UV fingerprints of three species of original plants of polygonati rhizoma had fingerprint characteristics. Their UV spectra were significantly different at 210 nm, 220 nm and 280 nm, and the three-dimensional projection of cluster analysis and principal component analysis showed that the accumulation of chemical components in different species was different. The differences among three species could be distinguished by HCA and PCA. Three species of polygonati rhizoma original plants, i.e. Polygonatum kingianum, P. sibiricum and P. cyrtonema, could be identified rapidly by UV fingerprint with chemometrics. That can provide an auxiliary method for clinical application, resource development and classification of Polygonatum.

Cite this article

杜泽飞,张 霞,温 翔,段宝忠 . Identification and Analysis of Original Plants of Polygonati Rhizoma by UV Fingerprint Combined with Chemometrics[J]. Subtropical Plant Science, 2022 , 51(2) : 125 -130 . DOI: 10.3969/j.issn.1009-7791.2022.02.006

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