Yunnan wild Dendrobium trigonopus seed plantlets in vitro aseptic germination was used as test material to explore the tissue culture and rapid propagation techniques of D. trigonopus. The results showed that the culture medium of B5 + NAA 0.5 mg·L^-1 + 10% potato juice and B5 + NAA 0.5 mg·L^-1 + 6-BA 0.5 mg·L^-1 + 10% potato juice had a significant effect to seedlings germination and protocorm proliferation of D. trigonopus respectively; the best rooting medium was B5 + NAA 0.5 mg·L^-1+ 10% potato juice + bark powder 2 g·L^-1. Seedlings could be directly transplanted in the matrix of sawdust and wood shaving or sawdust and bark each half when they were simply sterilized, the survival rate exceeded 95% after transplanted 25 days.