Stems with axillary buds from the elite of Anoectochilus roxburghii (Wall.) Lindl, were used as explants for in vitro rapid propagation. The results showed that the best culture media was 1/2MS+6-BA 1.5 mg·L^-1 + NAA 0.2 mg·L^-1; the proliferation culture media of was 1/2MS +6-BA 2.0 mg·L^-1 + NAA 0.5 mg·L^-1 + banana 100 g·L^-1, the proliferation coefficient was 6 ~ 8 times; the rooting culture media was 1/2MS + IBA 1.0 mg·L^-1 +NAA 0.5 mg·L^-1+AC (activated charcoal) 0.2 g·L^-1, the rooting coefficient was 95 percent. The rooting seedling transplanted into the mixture mixed by stone and cinder, the drainage ability was good, cover the film to keep Moisturizing, with a success rate of around 90 percent.