Plant physiology, biochemistry and molecular biology

Identification of Chemical Components of Acanthopanax trifoliatus by UPLC-Q-Orbitrap HRMS

  • 陈兰青,黎淑贞,甘礼社,金静维,吴日辉,马 航,张 焜,李冬利
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  • (School of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529000, Guangdong China)

Received date: 2023-04-17

  Accepted date: 2023-06-25

  Online published: 2023-11-28

Abstract

An ultra-high performance liquid chromatography-quadrupole/electrostatic field-orbital-trap-high resolution mass spectrometry (UPLC-Q-Orbitrap HRMS) method for the analysis of various chemical components in Acanthopanax trifoliatus was established. The sample was separated by Hypersil GOLD C18 column (100 mm × 2.1 mm, 1.9 μm), eluting with 0.1% formic acid acetonitrile-0.1% formic acid aqueous solution. Chemical components were quickly identified by mass spectrometry and comfirmed by the negative ion full scan mode and the secondary mass spectrometer automatic trigger scan. The chemical components were identified by database, literature comparison and reference materials. A total of 29 chemical components were identified, including nineteen polyphenolic compounds and ten terpenoids. This study was the first to report the feasibility of using the UPLC-Q-Orbitrap HRMS method to quickly and comprehensively analyze the active chemical components of A. trifoliatus. The method can provide a basis for the analysis of active chemical composition of A. trifoliatus.

Cite this article

陈兰青,黎淑贞,甘礼社,金静维,吴日辉,马 航,张 焜,李冬利 . Identification of Chemical Components of Acanthopanax trifoliatus by UPLC-Q-Orbitrap HRMS[J]. Subtropical Plant Science, 2023 , 52(4) : 301 -309 . DOI: 10.3969/j.issn.1009-7791.2023.04.004

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