Research articles

Identification and Expression Analysis of PYL Gene Family in Eriobotrya japonica

  • 林秋香,韩雨昕
Expand
  • (Meizhouwan Vocational Technology College, Putian 351119, Fujian China)

Received date: 2024-01-29

  Accepted date: 2024-02-18

  Online published: 2024-04-03

Abstract

Abscisic acid (ABA) is one of the important hormones regulating plant growth and development, and abiotic stress. As an ABA receptor, PYL is located at the top of its signaling pathway and plays a core regulatory role. However, this gene family has not been identified and analyzed in detail in loquat. In this study, 12 PYL genes were identified in loquat based on bioinformatics analysis. The molecular weight of the coding PYL protein ranged from 10.47 to 29.12 kDa, and most of them were localized in the cytoplasm. PYL family genes were distributed on 8 chromosomes. Phylogenetic tree analysis showed that PYL gene family could be divided into three subfamilies and EjPYL1 was closely related to AtPYL13. PYL proteins in loquat were consisted of motif 1-6, and motif3 was the common domain. Genes of PYL family were collinearity in species loquat. There were 2 pairs of collinearity between loquat and Arabidopsis thaliana. Cis-components in promoter of PYL genes were related to low temperature, drought, light and hormone response. RNA-seq of loquat young fruits indicated that EjPYL6 and EjPYL8 were both up-regulated by low temperature in fruit pulp and seeds. This study can provide theoretical basis for further understanding ABA signal transduction pathway and breeding new varieties of loquat with frost resistance.

Cite this article

林秋香,韩雨昕 . Identification and Expression Analysis of PYL Gene Family in Eriobotrya japonica[J]. Subtropical Plant Science, 2024 , 53(1) : 1 -11 . DOI: 10.3969/j.issn.1009-7791.2024.01.001

References

[1] 顾林平, 张冰, 费艳, 王化坤, 王鹏凯. 枇杷iPBS分子标记的PCR体系优化与引物筛选[J]. 中国南方果树, 2019, 48(1): 41–46.
[2] 谢钟琛, 李健. 早钟6号枇杷幼果冻害温度界定及其栽培适宜区区划[J]. 福建果树, 2006(1): 7–11.
[3] 林顺权. 枇杷属野生种种质资源的研究与创新利用进展[J]. 园艺学报, 2017, 44(9): 1704–1716.
[4] 李晓颖, 徐红霞, 陈俊伟. 枇杷WRKY转录因子鉴定与表达分析[J]. 园艺学报, 2019, 46(5): 939–954.
[5] 郭云, 王铁梅. 基于RNA-Seq技术的苜蓿根蘖性状发生相关下调基因[J]. 草业科学, 2016, 33(1): 75–85.
[6] 郭贵华, 刘海艳, 李刚华, 刘明, 李岩石. ABA缓解水稻孕穗期干旱胁迫生理特性的分析[J]. 中国农业科学, 2014, 47(22): 4380–4391.
[7] 李薇, 史菲, 刘敏, 陈水森. 植物响应低温的生理和分子机制研究进展[J]. 北方园艺, 2023(8): 121–126.
[8] 刘丽杰, 苍晶, 于晶, 王健飞, 黄儒, 徐琛, 张成亮. 外源ABA对冬小麦越冬期蔗糖代谢的影响[J]. 植物生理学报, 2013, 49(11): 1173–1180.
[9] 辛慧慧, 李防洲, 侯振安, 冶军, 康文晶, 罗建. 低温胁迫下棉花幼苗对外源水杨酸的生理响应[J]. 植物生理学报, 2014, 50(5): 660–664.
[10] Inupakutika M, Devireddy A R, Willmon D, Puppala N, Cho Y. Genome-wide comparative analysis of genes encoding core components of ABA signaling pathway in legume family [J]. International Journal of Computational Bioinformatics and In Silico Modeling, 2016, 5(4): 828–843.
[11] Melcher K, Ng L M, Zhou X E, Soon F F, Xu Y, Suino-Powell K M, Park S Y, Weiner J J, Fujii H, Chinnusamy V. A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors [J]. Nature, 2009: 536–608.
[12] Szostkiewicz I, Richter K, Kepka M, Demmel S, Ma Y, Korte A, Assaad F F, Christmann A, Grill E. Closely related receptor complexes differ in their ABA selectivity and sensitivity [J]. 2010, 61(1): 25–35.
[13] Santiago J, Dupeux F, Round A, Antoni R, Park S Y, Jamin M, Cutler S R, Rodriguez P L, Marquez J A. The abscisic acid receptor PYR1 in complex with abscisic acid [J]. Nature, 2009, 462(7273): 665–668.
[14] Ma Y, Szostkiewicz I, Korte A, Moes D, Alexander Y. Regulators of PP2C phosphatase activity function as abscisic acid sensors [J]. Science, 2009, 324(5930): 1064–1068.
[15] Cutler S R, Rodriguez P L, Finkelstein R R, Abrams S. Abscisic acid: emergence of a core signaling network [J]. Annual Review of Plant Biology, 2010, 61(1): 651–679.
[16] 马宗桓, 陈佰鸿, 李文芳, 毛娟. 葡萄PYL基因家族的鉴定与表达分析[J]. 果树学报, 2018, 35(3): 265–274.
[17] Pizzio G A, Rodriguez L, Antoni R, Gonzalez-Guzman M, Yunta C, Merilo E, Kollist H, Albert A, Rodriguez P L. The PYL4 A194T mutant uncovers a key role of PYR1–LIKE 4/PROTEIN PHOSPHATASE 2CA interaction for abscisic acid signaling and plant drought resistance [J]. Plant Physiology, 2013, 163(1): 441–455.
[18] Xing L, Zhao Y, Gao J H, Xiang C B, Zhu J K. The ABA receptor PYL9 together with PYL8 plays an important role in regulating lateral root growth [J]. Scientific Reports, 2016, 6(1): 27176–27177.
[19] He Z H, Zhong J W, Su X P, Wang B C, William T, Dai M Q. The maize ABA receptors ZmPYL8, 9, and 12 facilitate plant drought resistance [J]. Frontiers in Plant Science, 2018, 9: 421–422.
[20] Hyunmi K, Kyeyoon L, Hyunsik H, Nikita B, Kim D Y, Sun Y I, Byun M O, Kim S T, Jung K H, Kim B G. Over expression of PYL5 in rice enhances drought tolerance, inhibits growth, and modulates gene expression [J]. Journal of Experimental Botany, 2014: 453–464.
[21] 赵雷. 植物脱落酸受体PYR/PYL/RCAR的研究进展[J]. 湖北农业科学, 2019, 58(18): 14–18.
[22] 熊亦凡. 枇杷钙感受器基因家族的鉴定及其在冻害胁迫下的表达与功能研究[D]. 福州: 福建农林大学硕士学位论文, 2022: 1–99.
[23] 黄思源, 呼天明, 杨培志. 蒺藜苜蓿PYL基因家族的全基因组鉴定、表达和功能分析[J]. 草业科学, 2019, 36(2): 422–433.
[24] Parks S Y, Fung P, Nishimura N, Jensen D R, Fujii H, Zhao Y, Lumba S, Santiago J, Rodrigues A, Cutler S R. Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins [J]. Science, 2009, 324(5930): 1068–1071.
[25] 王雨璇, 徐晨, 李桂新, 丁忠杰, 郑绍建. PP2C蛋白磷酸酶家族参与植物多种胁迫应答的研究进展[J]. 植物生理学报, 2023, 59(8): 1463–1473.
[26] 周玉萍, 颜嘉豪, 田长恩. 保卫细胞中ABA信号调控机制研究进展[J]. 植物学报, 2022, 57(5): 684–696.
[27] 王慧, 汲宁宁, 钱关泽. 小麦脱落酸(ABA)信号传导途径研究进展[J]. 聊城大学学报(自然科学版), 2022, 35(5): 94–102.
[28] Park S Y, Peterson F C, Mosquna Y, Volkman B F, Cutler S R. Agrochemical control of plant water use using engineered abscisic acid receptors [J]. Nature, 2015, 520(7548): 545–548.
[29] 王傲雪, 孟令君, 陈秀玲, 莫福磊, 吕瑞, 薛小棚, 孟凡越, 齐浩楠, 张珍珠. 番茄脱落酸受体PYL基因家族全基因组鉴定及表达分析[J]. 东北农业大学学报, 2023, 54(1): 21–32.
[30] 贾小霞, 齐恩芳, 马胜, 黄伟, 郑永伟, 白永杰, 文国宏. 马铃薯PYL基因家族的全基因组鉴定及表达分析[J]. 作物学报, 2022, 48(10): 2533–2545.
Outlines

/

〈 〉