Review

Multi-omics Research Progress on the Active Ingredients Biosynthesis and Regulation in Dendrobium officinale

  • 刘黎卿,郭迟鸣,施建羽,郭 莺,孟红岩,曾新萍
Expand
  • (Fujian Institute of Subtropical Botany / Fujian Key Laboratory of Subtropical Plant Physiology and Biochemistry, Xiamen 361006, Fujian China)

Received date: 2023-12-13

  Accepted date: 2024-05-16

  Online published: 2024-11-20

Abstract

Dendrobium officinale, an important medicinal plant in the Dendrobium genus of Orchidaceae, is rich in active metabolites such as polysaccharides, alkaloids and flavonoids. The biosynthesis, regulation and accumulation of plant metabolites is a complex process. In recent years, omics technologies such as genomics, transcriptomics, proteomics, and metabolomics have developed rapidly, providing a deeper understanding of the multi-level regulatory network of D. officinale metabolites from different dimensions such as transcription, post transcription, translation, and epigenetics. This article reviews the multi-omics research progress on the active ingredients biosynthesis and regulation in D. officinale, and prospects the future research directions, which can provide theoretical basis for molecular breeding, cultivation management and industrial application of D. officinale.

Cite this article

刘黎卿,郭迟鸣,施建羽,郭 莺,孟红岩,曾新萍 . Multi-omics Research Progress on the Active Ingredients Biosynthesis and Regulation in Dendrobium officinale[J]. Subtropical Plant Science, 2024 , 53(4) : 381 -388 . DOI: 10.3969/j.issn.1009-7791.2024.04.013

References

[1] Xu X, Zhang C, Wang N, Xu Y, Tang G, Xu L, Feng Y. Bioactivities and mechanism of actions of Dendrobium officinale: A comprehensive review [J]. Oxidative Medicine and Cellular Longevity, 2022: 6293355.
[2] Yang J, Kuang M T, Yang L, Huang W, Hu J M. Modern interpretation of the traditional application of Shihu – A comprehensive review on phytochemistry and pharmacology progress of Dendrobium officinale [J]. Journal of Ethnopharmacology, 2023, 302(Pt A): 115912.
[3] Ng T B, Liu J Y, Wong J H, Ye X J, Wing Sze S C, Tong Y, Zhang K Y. Review of research on Dendrobium, a prized folk medicine [J]. Applied Microbiology and Biotechnology, 2012, 93(5): 1795–1803.
[4] Zhao Y, Liu G Z, Yang F, Liang Y L, Gao Q Q, Xiang C F, Li X, Yang R, Zhang G H, Jiang H F, Yu L, Yang S C. Multilayered regulation of secondary metabolism in medicinal plants [J]. Molecular Horticulture, 2023, 3(1): 11.
[5] 梁浩, 孙海, 钱佳奇, 张亚玉. 药用植物代谢调控的组学研究进展[J]. 中药材, 2023(8): 2085–2092.
[6] Wang Y, Tong Y, Adejobi O I, Wang Y H, Liu A Z. Research advances in multi-omics on the traditional chinese herb Dendrobium officinale [J]. Frontiers in plant science, 2022(12): 808228.
[7] He Y, Li L, Chang H, Cai B, Gao H, Chen G, Hou W, Jappar Z, Yan Y. Research progress on extraction, purification, structure and biological activity of Dendrobium officinale polysaccharides [J]. Frontiers in Nutrition, 2022(9): 965073.
[8] 严静, 蔡易熹, 陈燕兰, 张慧莹, 杜冰. 铁皮石斛茎、叶、花的活性成分及综合利用研究进展[J]. 食品与发酵工业, 2021, 47(17): 299–306.
[9] Zhang P, Zhang X Y, Zhu X Y, Hua Y F. Chemical constituents, bioactivities, and pharmacological mechanisms of Dendrobium officinale: A review of the past decade [J]. Journal of Agricultural and Food Chemistry, 2023, 71(41): 14870–14889.
[10] Cao H, Ji Y L, Li S C, Lu L, Tian M, Yang W, Li H. Extensive metabolic profiles of leaves and stems from the medicinal plant Dendrobium officinale Kimura et Migo [J]. Metabolites, 2019, 9(10): 215.
[11] He C M, Zhang J X, Liu X C, Zeng S J, Wu K L, Yu Z M, Wang X J, Teixeira Da Silva J A, Lin Z J, Duan J. Identification of genes involved in biosynthesis of mannan polysaccharides in Dendrobium officinale by RNA-seq analysis [J]. Plant Molecular Biology, 2015, 88(3): 219–231.
[12] Wang Z J , Jiang W M, Liu Y Y, Meng X X, Su X L, Cao M Y, Wu L P, Yu N J, Xing S H, Peng D Y. Putative genes in alkaloid biosynthesis identified in Dendrobium officinale by correlating the contents of major bioactive metabolites with genes expression between Protocorm-like bodies and leaves [J]. BMC Genomics, 2021, 22(1): 579.
[13] Shen C J, Guo H, Chen H L, Shi Y J, Meng Y J, Lu J J, Feng S G, Wang H Z. Identification and analysis of genes associated with the synthesis of bioactive constituents in Dendrobium officinale using RNA-Seq [J]. Scientific Reports, 2017, 7(1): 187.
[14] Niu Z T, Zhu F, Fan Y J, Li C, Zhang B H, Zhu S Y, Hou Z Y, Wang M T, Yang J P, Xue Q Y, Liu W, Ding X Y. The chromosome-level reference genome assembly for Dendrobium officinale and its utility of functional genomics research and molecular breeding study [J]. Acta Pharmaceutica Sinica B, 2021, 11(7): 2080–2092.
[15] Yan L, Wang X, Liu H, Tian Y, Lian J M, Yang R J, Hao S M, Wang X J, Yang S C, Li Q Y, Qi S, Kui L, Okpekum M, Ma X, Zhang J J, Ding Z L, Zhang G L, Wang W, Dong Y, Sheng J. The genome of Dendrobium officinale illuminates the biology of the important traditional Chinese orchid herb [J]. Molecular Plant, 2015, 8(6): 922–934.
[16] Zhang G Q, Xu Q, Bian C, Tsai W C, Yeh C M, Liu K W, Yoshida K, Zhang L S, Chang S B, Chen F, Shi Y, Su Y Y, Zhang Y Q, Chen L J, Yin Y Y, Lin M, Huang H X, Deng H, Wang Z W, Zhu S L, Zhao X, Deng C, Niu S C, Huang J, Wang M N, Liu G H, Yang H J, Xiao X J, Hsiao Y Y, Wu W L, Chen Y Y, Mitsuda N, Ohme-Takagi M, Luo Y B, Van De Peer Y, Liu Z J. The Dendrobium catenatum Lindl. genome sequence provides insights into polysaccharide synthase, floral development and adaptive evolution [J]. Scientific Reports, 2016, 6: 19029.
[17] He L, Fu S F, Xu Z C, Yan J, Xu J, Zhou H, Zhou J G, Chen X L, Li Y, Au K F, Yao H. Hybrid sequencing of full-length cDNA transcripts of stems and leaves in Dendrobium officinale [J]. Genes, 2017, 8(10): 257.
[18] Zhang J X, He C M, Wu K L, Teixeira Da Silva J A, Zeng S J, Zhang X H, Yu Z M, Xia H Q, Duan J. Transcriptome analysis of Dendrobium officinale and its application to the identification of genes associated with polysaccharide synthesis [J]. Frontiers in Plant Science, 2016, 7: 5.
[19] Guo X, Li Y, Li C F, Luo H M, Wang L Z, Qian J, Luo X, Xiang L, Song J Y, Sun C, Xu H B, Yao H, Chen S L. Analysis of the Dendrobium officinale transcriptome reveals putative alkaloid biosynthetic genes and genetic markers [J]. Gene, 2013, 527(1): 131–138.
[20] Yuan Y D, Zhang J C, Liu X, Meng M J, Wang J P, Lin J. Tissue-specific transcriptome for Dendrobium officinale reveals genes involved in flavonoid biosynthesis [J]. Genomics, 2020, 112(2): 1781–1794.
[21] 林江波, 王伟英, 邹晖, 戴艺民. 基于转录组测序的铁皮石斛黄酮代谢途径及相关基因解析[J]. 福建农业学报, 2019, 34(9): 1019–1025.
[22] Ren Z Y, Qiu F N, Wang Y J, Yu W X, Liu C X, Sun Y Y, Wang Y W, Zhang X F, Xing S P, Tao S C, Huang Y C, Liu G X, Wei Z F, Yu B Y, Du S X, Lei Z X, Wei G. Network analysis of transcriptome and LC-MS reveals a possible biosynthesis pathway of anthocyanins in Dendrobium officinale [J]. BioMed Research International, 2020, 2020: 6512895.
[23] Lei Z X, Zhou C H, Ji X Y, Wei G, Huang Y C, Yu W X, Luo Y Y, Qiu Y. Transcriptome analysis reveals genes involved in flavonoid biosynthesis and accumulation in Dendrobium catenatum from different locations [J]. Scientific Reports, 2018, 8(1): 6373.
[24] Zhan X Q, Qi J F, Zhou B, Mao B Z. Metabolomic and transcriptomic analyses reveal the regulation of pigmentation in the purple variety of Dendrobium officinale [J]. Scientific Reports, 2020(1): 17700.
[25] Jiao C Y, Song C, Zheng S Y, Zhu Y P, Jin Q, Cai Y P, Lin Y. Metabolic profiling of Dendrobium officinale in response to precursors and methyl jasmonate [J]. International Journal of Molecular Sciences, 2018, 19(3): 728.
[26] Chen Y, Wang Y Z, Lyu P, Chen L P, Shen C J, Sun C B. Comparative transcriptomic analysis reveal the regulation mechanism underlying MeJA-induced accumulation of alkaloids in Dendrobium officinale [J]. Journal of Plant Research, 2019, 132(3): 419–429.
[27] Adejobi O I, Guan J, Yang L, Hu JM, Yu A, Muraguri S, Liu A. Transcriptomic analyses shed light on critical genes associated with bibenzyl biosynthesis in Dendrobium officinale [J]. Plants (Basel), 2021, 10(4): 633.
[28] Jiao C Y, Wei M K, Fan H H, Song C, Wang Z L, Cai Y P, Jin Q. Transcriptomic analysis of genes related to alkaloid biosynthesis and the regulation mechanism under precursor and methyl jasmonate treatment in Dendrobium officinale [J]. Frontiers in Plant Science, 2022, 13: 941231.
[29] Jia Y, Liu J, Xu M Y, Chen G H, Tan M P, Xiang Z X. Light and potassium improve the quality of Dendrobium officinale through optimizing transcriptomic and metabolomic alteration [J]. Molecules, 2022, 27(15): 4866.
[30] Li D X, Ye G Y, Li J, Lai Z Q, Ruan S Y, Qi Q, Wang Z H, Duan S J, Jin H L, Wang H B. High light triggers flavonoid and polysaccharide synthesis through DoHY5-dependent signaling in Dendrobium officinale [J]. The Plant Journal, 2023, 115(4): 1114–1133.
[31] Chen Y, Shen Q, Lv P, Sun C B. Comparative metabolomic analyses of Dendrobium officinale Kimura et Migo responding to UV-B radiation reveal variations in the metabolisms associated with its bioactive ingredients [J]. Peer J, 2020, 8: e9107.
[32] Li H S, Yang Y F, Ye W, Sun G. Exploration of the effect of blue laser light on microRNAs involved in functional metabolism in D. officinale through RNA sequencing [J]. Gene, 2023, 851: 147009.
[33] Yang Y F, Qiu Y Q, Ye W, Sun G, Li H S. RNA sequencing- based exploration of the effects of far-red light on microRNAs involved in the shade-avoidance response of D. officinale [J]. Peer J, 2023, 11: e15001.
[34] Li H S, Ye W, Wang Y Q, Chen X H, Fang Y, Sun G. RNA sequencing-based exploration of the effects of far-red light on lncRNAs involved in the shade-avoidance response of D. officinale [J]. Peer J, 2021, 9: e10769.
[35] Fang C, Xin G Z, Wang SL, Wei M M, Wu P, Dong X M, Song G Q, Xie T, Zhou J L. Discovery and validation of peptide biomarkers for discrimination of Dendrobium species by label-free proteomics and chemometrics [J]. Journal of Pharmaceutical and Biomedical Analysis, 2020, 182: 113118.
[36] Jin Q, Jiao C Y, Sun S W, Song C, Cai Y P, Lin Y, Fan H H, Zhu Y F. Metabolic analysis of medicinal Dendrobium officinale and Dendrobium huoshanense during different growth years [J]. PLoS ONE, 2016, 11(1): e0146607.
[37] Zhou D, Zhao Y, Chen Z L, Yan X X, Zhao Y G, Gao L, Yang L X. Traditional processing increases biological activities of Dendrobium offificinale Kimura et. Migo in Southeast Yunnan, China [J]. Scientific Reports, 2022, 12(1): 14814.
[38] Liu S, Zhang H Y, Yuan Y D. A Comparison of the flavonoid biosynthesis mechanisms of Dendrobium species by analyzing the transcriptome and metabolome [J]. International Journal of Molecular Sciences, 2022, 23(19): 11980.
[39] Yuan Y D, Zuo J J, Zhang H Y, Zu M T, Yu M Y, Liu S. Transcriptome and metabolome profiling unveil the accumulation of flavonoids in Dendrobium officinale [J]. Genomics, 2022, 114(3): 110324.
[40] Hu J, Huang W X, Zhang F T, Luo X D, Chen Y L, Xie J K. Variability of volatile compounds in the medicinal plant Dendrobium officinale from different regions [J]. Molecules, 2020, 25(21): 5046.
[41] Lan Q Q, Liu C X, Wu Z H, Ni C, Li J Y, Huang C L, Wang H, Wei G. Does the metabolome of wild-like Dendrobium officinale of different origins have regional differences? [J]. Molecules, 2022, 27(20): 7024.
[42] He Q Q, Lu A J, Qin L, Zhang Q R, Lu Y L, Yang Z, Tan D P, He Y Q. An UPLC–Q–TOF/MS-based analysis of the differential composition of Dendrobium officinale in different regions [J]. Journal of Analytical Methods in Chemistry, 2022, 2022: 8026410.
[43] Yang D, Song Y Y, Lu A J, Qin L, Tan D P, Zhang Q R, He Y Q, Lu Y L. Metabolomics-based analysis of the effects of different cultivation strategies on metabolites of Dendrobium officinale Kimura et Migo [J]. Metabolites, 2023, 13(3): 389.
[44] 李帆, 金传高, 任仙樱, 费璇, 赵祺, 潘晓军, 吴志刚, 姜程曦. 基于UPLC–MS/MS代谢组学技术分析不同栽培模式下铁皮石斛类黄酮化合物差异性[J]. 中草药, 2022, 53 (4): 1156–1162.
[45] Zuo S M, Yu H D, Zhang W M, Zhong Q P, Chen W X, Chen W J, Yun Y H, Chen H M. Comparative metabolomic analysis of Dendrobium officinale under different cultivation substrates [J]. Metabolites, 2020, 10(8): 325.
[46] Chen J, Wang L, Liang H, Jin X W, Wan J, Liu F, Zhao K, Huang J, Tian M L. Overexpression of DoUGP enhanced biomass and stress tolerance by promoting polysaccharide accumulation in Dendrobium officinale [J]. Frontiers in Plant Science, 2020, 11: 533767.
[47] 胡淞, 田英男, 冯垒, 兰晓天, 黄鑫, 师凯辉, 韩欣妤, 朱永平, 和凤美. 利用CRISPR/cas9技术研究铁皮石斛Csl4基因的功能[J]. 分子植物育种, 2021, 19(8): 2596–2602.
Outlines

/