Subtropical Plant Science >

2024 , Vol. 53 >Issue 5: 389 - 398

DOI: https://doi.org/10.3969/j.issn.1009-7791.2024.05.001

Research articles

Inoculation with AMF Promotes the Photosynthetic and Antioxidant Capacities of Ponkan Leaves under High Temperature and Drought Stress

  • 周宏蕙，葛 聪，黄晓冰，张 龙，卢聪慧，张宇平，王 平
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  • (1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China; 2. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China; 3 Tianma Citrus Orchard of Yongchun County, Quanzhou 362600, Fujian China)

Received date: 2024-04-22

  Accepted date: 2024-06-13

  Online published: 2025-01-25

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Abstract

The effects of inoculation with Arbuscular mycorrhizal fungi (AMF) on the photosynthesis and antioxidant capacity in the leaves of Citrus reticulata ‘Ponkan’ under high temperature and drought stress were investigated. This study would provide Ponkan a production measure for resisting high temperature and drought stress. The experimental treatments were designed as follows: (1) Ponkan without AMF inoculation under high temperature and drought stress; (2) Ponkan with AMF inoculation under high temperature and drought stress; (3) Ponkan without AMF treatment under normal environmental conditions (control). In this study, AMF infection rate in the root and phenotype of Ponkan were observed, and the photosynthetic parameters, reactive oxygen species (ROS) content and antioxidant enzyme activities were measured in the leaves. The results showed that the leaves of Ponkan without AMF inoculation were obviously yellowed and wilted under high temperature and drought stress compared with the control. The net photosynthetic rate, stomatal conductance, transpiration rate, water use efficiency, maximum photochemical efficiency, actual photosynthetic efficiency and photosynthetic electron transport efficiency in the leaves of Ponkan without AMF inoculation were significantly decreased under high temperature and drought stress, while the intercellular CO2 concentration was increased compared with those of control. However, the net photosynthetic rate and stomatal conductance were increased, the intercellular CO2 concentration was decreased, and the photosynthetic capacity was enhanced in the leaves of Ponkan with AMF inoculation compared with those of Ponkan without AMF inoculation under high temperature and drought stress. In addition, the contents of O2܋, H2O2 and malondialdehyde (MDA) in leaves of Ponkan without AMF inoculation were increased while the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and ascorbate peroxidase (APX) were decreased under high temperature and drought stress compared with those of control. However, the contents of O2܋, H2O2 and MDA were decreased and the activities of the antioxidant enzymes were increased in the leaves of Ponkan with AMF inoculation compared with those of Ponkan without AMF inoculation under high temperature and drought stress. The results demonstrated that Ponkan growth was inhibited, and the photosynthetic and antioxidant capacities was reduced in the the leaves of Ponkan without AMF inoculation, while the capacities were effectively improved in the leaves of Ponkan with AMF inoculation under high temperature and drought stress. Therefore, we conclude that the higher photosynthetic and ROS scavenging capacities in the leaves of Ponkan with AMF inoculation alleviate the physiological damage of the plants under high temperature and drought stress.

Key words： Ponkan; Arbuscular mycorrhizal fungi (AMF); high temperature and drought stress; photosynthesis; antioxidant enzymes; ROS

Cite this article

周宏蕙，葛 聪，黄晓冰，张 龙，卢聪慧，张宇平，王 平 . Inoculation with AMF Promotes the Photosynthetic and Antioxidant Capacities of Ponkan Leaves under High Temperature and Drought Stress[J]. Subtropical Plant Science, 2024 , 53(5) : 389 -398 . DOI: 10.3969/j.issn.1009-7791.2024.05.001

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