Subtropical Plant Science >

2024 , Vol. 53 >Issue 5: 416 - 424

DOI: https://doi.org/10.3969/j.issn.1009-7791.2024.05.004

Research articles

Full-length Transcriptome Sequencing Analysis of Medicinal Plant Pithecellobium clypearia

  • 钟凤娣，凌腾泓，牟桂萍，朱孔宇，王鹏龙，闫晓东
Expand
  • (1. Guangdong Lingnanyuan Exploration and Design Co., Ltd, Guangzhou 510095, Guangdong China; 2. College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, Guangdong China; 3. Guangdong Provincial Key Laboratory of Intelligent Port Security Inspection, Guangzhou 510700, Guangdong China; 4. College of Environment and Chemical Engineering, Foshan University, Foshan 528000, Guangdong China; 5. Technology Center of Huangpu Custom, Guangzhou 510730, Guangdong China)

Received date: 2024-07-12

  Accepted date: 2024-08-13

  Online published: 2025-01-25

Fold

Abstract

As the plant with remarkable medicinal value, the insufficiency of genomic information of Pithecellobium clypearia restricts its in-depth comprehension. In this study, PacBio Sequel sequencing technology was employed to conduct full-length transcriptome sequencing of the mixed samples from roots, mature branches, young branches, mature leaves, and young leaf tissues of P. clypearia. The results showed that 118 472 unique transcripts were successfully identified and further categorized into 44 939 Unigenes. After comparison with the Nr, Uniprot, GO, KEGG, and KOG databases, functional annotations were provided for 37 198 Unigenes, and the majority of these Unigenes were involved in biological processes and metabolic pathways. Simultaneously, 13 897 Unigenes were discovered to undergo alternative splicing, including intron retention, 3' end splicing diversity, and 5' end splicing diversity. Additionally, 377 and 159 structural genes involved in the biosynthesis of flavonoids and terpenoids, as well as 132 and 180 Unigenes encoding members of the MYB and WRKY transcription factor families, were identified. This study expanded the genomic resources of P. clypearia, provided valuable data support for exploring the metabolic regulatory mechanism of its bioactive components, screening of key genes, and development of molecular markers, laid a foundation for further exploitation of the medicinal value of P. clypearia in future.

Key words： Pithecellobium clypearia; full-length transcriptome; flavonoid; terpenoid; alternative splicing

Cite this article

钟凤娣，凌腾泓，牟桂萍，朱孔宇，王鹏龙，闫晓东 . Full-length Transcriptome Sequencing Analysis of Medicinal Plant Pithecellobium clypearia[J]. Subtropical Plant Science, 2024 , 53(5) : 416 -424 . DOI: 10.3969/j.issn.1009-7791.2024.05.004

References

[1] 付姝颖, 潘超美, 刘良婷, 张家瑛, 苏家贤, 柳亚青, 杨森山, 邹国光. 药用植物猴耳环的组织培养与快速繁殖[J]. 植物生理学报, 2015, 51(12): 2195–2200. 

[2] 李梅, 黄世能, 陈祖旭, 马星宇, 金文云. 药用乔木树种猴耳环研究现状及开发利用前景[J]. 林业科学, 2018, 54(4): 142–155. 

[3] 马学忠, 蓝芳荣, 黄建, 陈冬秀, 何源福. 猴耳环南岭北部引种试验[J]. 林业科技通讯, 2022, 12: 67–69. 

[4] 付元风, 刘芳, 李思佳, 潘卫松, 张婷. 猴耳环的研究进展[J]. 中草药, 2018, 49(5): 1174–1183. 

[5] 范文昌, 梅全喜. 广东地产清热解毒药物大全[M]. 北京: 中医古籍出版社, 2011. 

[6] 黄燮才. 中国民间生草药原色图谱[M]. 南宁: 广西科学技术出版社, 1994. 

[7] 彭亮, 李诒光, 陈杰, 饶毅, 魏惠珍. 我国猴耳环属植物药理作用及临床应用研究进展[J]. 江西中医药大学学报, 2015, 27(6): 117–120. 

[8] Barrera N L L, Bautista B S, Jimenez E M, Reyes C R. Influence of leaf, fruit and seed powders and extracts of Pithecellobium dulce (Roxb.) Benth (Fabaceae) on the in vitro vegetative growth of seven postharvest fungi [J]. Revista Mexicana de Fitopatología, 2002, 20: 66–71. 

[9] Katekhaye S D, Kale M S. Antioxidant and free radical scavenging activity of Pithecellobium dulce (Roxb.) Benth wood bark and leaves [J]. Free Radicals and Antioxidants, 2012, 3(2): 47–57. 

[10] Kang J, Liu C, Wang H Q, Li B M, Li C, Chen R Y, Liu A L. Studies on the bioactive flavonoids isolated from Pithecellobium clypearia Benth [J]. Molecules (Basel, Switzerland), 2014, 19(4): 4479–90. 

[11] 李伟, 颜兵. 猴耳环消炎颗粒治疗急性肠胃炎60例[J]. 实用中西医结合临床, 2011, 11(5): 57. 

[12] 王家蔚, 郁峰. 猴耳环消炎颗粒治疗急性上呼吸道感染疗效观察[J]. 实用中西医结合临床, 2011(3): 64–65.

[13]  兰小玲, 黄跃, 杨广林. 猴耳环消炎片治疗咽喉炎、扁桃腺炎200例疗效观察[J]. 实用临床医学, 2007, 8(2): 89–92.

[14]  秦双双, 周鹏程, 胡营, 杨晓男, 梁莹, 韦范, 林杨, 韦坤华, 缪剑华. 菝葜全长转录组测序及分析[J]. 中国现代中药, 2023, 25(7): 1451–1462.

[15]  Shan T Y, Xu J Y, Zhong X X, Zhang J J, He B, Tao Y J, Wu J W. Full-length transcriptome sequencing provides new insights into the complexity of flavonoid biosynthesis in Glechoma Longituba [J]. Physiologia Plantarum, 2023, 175(6): 14104.

[16] 田韦韦, 严志祥, 王成, 袁权, 华桦, 刘俐, 余冬梅, 王剑波, 赵军宁. 基于全长转录组测序的多花黄精WRKY转录因子家族分析[J]. 中国中药杂志, 2023, 48(4): 939–950.

[17]  黄丽容, 欧夏莲, 李翠, 胡营, 韦筱媚, 郭晓云, 雷明, 缪剑华. 马鞭草全长转录组测序分析[J]. 中国现代中药, 2023, 25(7): 1486–1495.

[18]  Ju Z G, Liang L, Zheng Y Q, Shi H X, Zhao W X, Sun W, Pang Y X. Full length transcriptome sequencing and RNA-seq analysis offer insights into terpenoid biosynthesis in Blumea balsamifera (L.) DC [J]. Gene, 2024, 15(3): 285.

[19]  Xu Z C, Peters R J, Weirather J, Luo H M, Liao B S, Zhang X, Zhu Y J, Ji A J, Zhang B, Hu S N, Au K F, Song J Y, Chen S L. Full-length transcriptome sequences and splice variants obtained by a combination of sequencing platforms applied to different root tissues of Salvia miltiorrhiza and tanshinone biosynthesis [J]. The Plant Journal, 2015, 82(6): 951–961.

[20]  Li J, Harata-Lee Y, Denton M D, Feng Q J, Rathjen J R, Qu Z P, Adelson D L. Long read reference genome-free reconstruction of a full-length transcriptome from Astragalus membranaceus reveals transcript variants involved in bioactive compound biosynthesis [J]. Cell Discovery, 2017, 3: 17031.

[21]  谭志强, 黄炬峰, 李福明, 邓乐平, 毛积鹏. 药用植物猴耳环的转录组测序分析[J]. 林业与环境科学, 2021, 37(3): 15–21.

[22]  黄婷, 彭冠明, 林昌明, 司徒荣贵, 毛积鹏. 猴耳环嫩枝和叶组织的代谢物分析[J]. 林业与环境科学, 2021, 37(4): 33–39.

[23]  李晓燕, 周瑾, 杜佳庚. 转录因子调控植物挥发性有机物生物合成的研究进展[J]. 四川农业大学学报, 2024, 42(2): 264–276.

Options
Outlines

/

〈 〉

Copyright © Editorial office of Subtropical Plant Science
Tel: 0592-5654157　E-mail: yrdzwkx@126.com
Supported by Beijing Magtech Co., Ltd.