In order to obtain efficient tissue cultural propagation technology of blueberry, the semi-lignification shoots of blueberry (Britewell) used as explants, propagation medium, rooting culture medium was selected. The result showed that 1/2MS＋2.0 mg·L-1 ZT＋0.01 mg·L-1 NAA＋30 g·L-1 sugar＋6.5 g·L-1 agar＋pH 5.8 is the optimal propagation medium to blueberry (Britewell), has 87% rate of buds induction on aseptic seeding, and the coefficient multiplication is 3.6，Improved ZT concentration, more callus emerged in propagation medium. its contributed to improving multiplication but also caused more thin aseptic seeding; 1/2 MS＋1.5 mg·L-1 IBA＋0.01 mg·L-1 NAA＋25 g·L-1 sugar＋7 g·L-1 agar＋pH 5.8 is the optimal rooting medium, has 70% rooting percentage. Being acclimatized for 10 d, the rooted plantlets were transplanted to turfy-soil and the survival rate was 78%. Control callus produced and cheaper hormones applicated is the key of efficient tissue cultural propagation technology of blueberry.
TAO Jun-feng
,
LI Ye-fang
,
SONG Jie
,
GUAN Wen-ling
. Tissue Culture Techniques of Blueberry (Britewell)[J]. Subtropical Plant Science, 2014
, 43(02)
: 159
-163
.
DOI: 10.3969/j.issn.1009-7791.2014.02.016
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