Research articles

Screening of Selenium-enriched Dendrobium Endophytes and Its Dynamic Changes of Selenium Enrichment

  • 刘如振,徐 炜,骆祝华,郭迟鸣,王 杰,林文珍
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  • (1. Fujian Institute of Subtropical Botany / Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Xiamen 361006, Fujian China; 2. Key Laboratory of Marine Biogenetic Resource, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, Fujian China)

Received date: 2024-07-04

  Accepted date: 2024-11-07

  Online published: 2025-03-20

Abstract

This study aimed to identify and characterize strains from Dendrobium officinale with high selenium tolerance and enrichment capacity, and to investigate the dynamics of selenium accumulation in these strains. The findings are intended to provide a theoretical foundation for the improvement of selenium-enriched microbial germplasm and for enhancing selenium content in plants. The strain BK-1 was initially identified through 16S rDNA sequence analysis. The strain’s tolerance to sodium selenite was then evaluated, determining its maximum tolerance concentration. The ability of the strain to convert inorganic selenium into organic selenium during the enrichment process was assessed, along with measurements of total selenium content, organic selenium yield, and conversion efficiency. An optimization experiment was also conducted to establish the optimal selenium ion concentration for supplementation. Strain BK-1 was identified as Burkholderia gladioli. This strain demonstrated a high tolerance to sodium selenite, with a maximum tolerance concentration of 130 μg·mL–1. During the selenium enrichment process, strain BK-1 achieved a total selenium content of 577.74 μg·g–1 DCW (dry cell weight ) within 48 hours, with an organic selenium yield of 505.66 μg·g–1 DCW and a conversion efficiency of 87.42%. The addition of selenium ions significantly shortened the logarithmic growth phase and reduced biomass, with the optimal selenium ion concentration determined to be 50 μg·mL–1.

Cite this article

刘如振,徐 炜,骆祝华,郭迟鸣,王 杰,林文珍 . Screening of Selenium-enriched Dendrobium Endophytes and Its Dynamic Changes of Selenium Enrichment[J]. Subtropical Plant Science, 2024 , 53(6) : 537 -544 . DOI: 10.3969/j.issn.1009-7791.2024.06.006

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