Research articles

Ultrasound-Assisted Deep Eutectic Solvent Extraction of Gymnema sylvestre Polysaccharides and Its Activity Determination

  • 郝胜杰,后海庆,尹 杰,李洪森,杜雨欣,靳枫阳,李 欠,陈桂林
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  • (1. College of Agriculture / State Key Laboratory of Crop Stress Biology for Arid Areas, Gansu Agricultural University, Lanzhou 730070, Gansu China; 2. Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, Hubei China)

Received date: 2024-09-15

  Accepted date: 2024-10-15

  Online published: 2025-03-20

Abstract

Optimization of ultrasonic-assisted deep eutectic solvents (DESs) extraction process for Gymnema sylvestre polysaccharides was conducted to investigate its biological activity, to provide theoretical support for the development of G. sylvestre products. Using the polysaccharide extraction yield as the evaluation index, the Box-Behnken response surface method was applied to optimize the extraction process based on single-factor experiments. Additionally, the antioxidant activities of the polysaccharides were evaluated by their ability to scavenge DPPH, ABTS+, FRAP, and H2O2 radicals, while the inhibitory effects on α-amylase and α-glucosidase, as well as antibacterial activity against several bacteria strains, were also studied. The results showed that the DESs synthesized from choline chloride and citric acid achieved the highest extraction rate. After optimization using the response surface method, under conditions where the molar ratio of choline chloride to citric acid was 1:1.02, water content was 39.32%, liquid-to-material ratio was 20.21:1, ultrasonic time was 39.23 min, ultrasonic temperature was 41.35 ℃, and ultrasonic power was 412 W, the extraction yield of G. sylvestre polysaccharides reached 29.23%, which was nearly 15 times higher than the traditional water extraction method. In vitro activity experiments demonstrated that G. sylvestre polysaccharides exhibited high antioxidant activity, broad-spectrum antibacterial capacity, and enzyme inhibition activity, showing great potential for developing novel anti-diabetic products. DESs, being environmentally friendly, efficient, and reusable as extraction media for natural products, significantly improved the extraction efficiency of G. sylvestre polysaccharides.

Cite this article

郝胜杰,后海庆,尹 杰,李洪森,杜雨欣,靳枫阳,李 欠,陈桂林 . Ultrasound-Assisted Deep Eutectic Solvent Extraction of Gymnema sylvestre Polysaccharides and Its Activity Determination[J]. Subtropical Plant Science, 2024 , 53(6) : 545 -553 . DOI: 10.3969/j.issn.1009-7791.2024.06.007

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